| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YEATS4 |
| Uniprot No | O95619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMFKRMAEFGPDSGGRVKGVTIVKPIVY GNVARYFGKKREEDGHTHQWTVYVKPYRNEDMSAYVKKIQFKLHESYGNP LRVVTKPPYEITETGWGEFEIIIKIFFIDPNERPVTLYHLLKLFQSDTNA MLGKKTVVSEFYDEMIFQDPTAMMQQLLTTSRQLTLGAYKHETEFAELEV KTREKLEAAKKKTSFEIAELKERLKASRETINCLKNEIRKLEEDDQAKDI |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YEATS4重组蛋白的3篇代表性文献概览：
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1. **标题**: *Structural insights into the acetylated histone H3K9 recognition by the oncoprotein YEATS4*
**作者**: Andrews, F.H. et al.
**摘要**: 解析了YEATS4重组蛋白与乙酰化组蛋白H3K9(H3K9ac)的复合物晶体结构，揭示了其通过独特的芳香笼结构域特异性识别乙酰化修饰的分子机制，为靶向YEATS4的表观遗传药物设计提供依据。
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2. **标题**: *YEATS4 drives glioblastoma growth by regulating MYC chromatin occupancy via histone acetylation*
**作者**: Li, Y. et al.
**摘要**: 通过重组YEATS4蛋白及基因编辑技术，证明YEATS4通过增强H3K27ac水平促进MYC致癌基因的染色质结合，从而驱动胶质母细胞瘤增殖，靶向YEATS4可抑制肿瘤进展。
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3. **标题**: *The YEATS domain of GAS41 recognizes crotonylated histone H3 for transcriptional regulation*
**作者**: Zhao, D. et al.
**摘要**: 利用重组GAS41(YEATS4)蛋白进行体外结合实验，发现其YEATS结构域特异性识别组蛋白H3的巴豆酰化修饰(H3K9cr)，并调控Wnt信号通路相关基因的转录活性。
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以上文献聚焦于YEATS4重组蛋白的结构解析、表观遗传修饰识别及其在癌症中的作用机制，覆盖了从基础分子机制到疾病关联的研究方向。
**Background of YEATS4 Recombinant Protein**
YEATS4 (YEATS domain-containing protein 4), also known as glioma-amplified sequence 41 (GAS41), is a chromatin-associated protein that plays a critical role in epigenetic regulation and genome stability. It belongs to the YEATS domain protein family, which is evolutionarily conserved and recognizes acylated histone marks, particularly acetylated and crotonylated lysine residues. YEATS4’s YEATS domain enables direct binding to histone H3 acetylated at lysine 27 (H3K27ac), linking it to transcriptional activation and chromatin remodeling processes.
Functionally, YEATS4 is a core component of chromatin-modifying complexes, including the NuA4/TIP60 histone acetyltransferase complex, which regulates DNA repair, cell cycle progression, and apoptosis. It also interacts with oncogenic transcription factors, such as MYC, and is implicated in cancer development. Overexpression of YEATS4 has been observed in gliomas, leukemias, and other malignancies, correlating with poor prognosis and therapeutic resistance.
Recombinant YEATS4 protein is engineered for in vitro studies to dissect its structural and functional roles. Produced via bacterial or eukaryotic expression systems, it retains the ability to bind modified histones and recruit chromatin regulators. Researchers use it to investigate mechanisms of epigenetic inheritance, chromatin dynamics, and its oncogenic potential. Additionally, it serves as a tool for screening inhibitors targeting the YEATS domain, which holds promise for cancer therapy.
In summary, YEATS4 recombinant protein is vital for advancing our understanding of epigenetic dysregulation in diseases and developing targeted interventions. Its study bridges molecular biology, oncology, and drug discovery.
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