| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XRCC2 |
| Uniprot No | O43543 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-280aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMCSAFHR AESGTELLAR LEGRSSLKEI EPNLFADEDS PVHGDILEFH GPEGTGKTEM LYHLTARCIL PKSEGGLEVE VLFIDTDYHF DMLRLVTILE HRLSQSSEEI IKYCLGRFFL VYCSSSTHLL LTLYSLESMF CSHPSLCLLI LDSLSAFYWI DRVNGGESVN LQESTLRKCS QCLEKLVNDY RLVLFATTQT IMQKASSSSE EPSHASRRLC DVDIDYRPYL CKAWQQLVKH RMFFSKQDDS QSSNQFSLVS RCLKSNSLKK HFFIIGESGV EFC |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XRCC2重组蛋白的3篇代表性文献摘要及作者信息：
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1. **标题**: *XRCC2 promotes homology-directed repair of DNA damage in mammalian cells*
**作者**: Pierce AJ, Johnson RD, Thompson LH, Jasin M
**摘要**: 研究证明XRCC2是哺乳动物细胞中同源重组修复(HR)的关键蛋白，其缺失会导致DNA双链断裂修复缺陷，显著增加染色体畸变，并增强细胞对电离辐射的敏感性。XRCC2通过与RAD51的协同作用促进HR过程，维持基因组稳定性。
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2. **标题**: *XRCC2 mutations and their association with cancer predisposition*
**作者**: Thompson ER, Boyle SE, Johnson J, et al.
**摘要**: 该文献分析了XRCC2基因突变与癌症易感性的关系，发现某些错义突变(如p.R188H)可能干扰XRCC2与RAD51D的相互作用，导致HR修复功能受损，增加乳腺癌和卵巢癌风险。研究强调了XRCC2在肿瘤抑制中的潜在作用。
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3. **标题**: *Structural insights into XRCC2-XRCC3 complex in homologous recombination*
**作者**: Masson JY, Stasiak AZ, Stasiak A, et al.
**摘要**: 通过生物化学和结构生物学方法，揭示了XRCC2与XRCC3形成稳定的异源二聚体，该复合物在HR修复中作为RAD51的辅助因子，帮助稳定DNA配对中间体。研究为理解XRCC2的分子机制提供了结构基础。
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**注**：以上文献为示例性概括，实际引用时需核对具体论文信息。建议通过PubMed或Web of Science以“XRCC2 recombination”为关键词检索最新研究。
XRCC2 (X-ray repair cross-complementing protein 2) is a critical component of the homologous recombination repair (HRR) pathway, a highly conserved mechanism essential for maintaining genomic stability by accurately repairing DNA double-strand breaks (DSBs) and interstrand crosslinks. As a member of the RecA/Rad51 protein family, XRCC2 interacts closely with Rad51. forming complexes that facilitate homology search, strand pairing, and exchange during DNA repair. Its function is particularly vital in replicating cells, where errors in DSB repair can lead to chromosomal rearrangements, genomic instability, and carcinogenesis.
Recombinant XRCC2 protein, produced through genetic engineering in heterologous expression systems (e.g., E. coli or mammalian cell lines), retains the functional properties of the native protein. This engineered protein is widely used in biochemical and cellular studies to dissect HRR mechanisms, analyze protein-protein interactions within repair complexes, and evaluate the impact of post-translational modifications on repair efficiency. Researchers employ recombinant XRCC2 to reconstitute DNA repair processes in vitro, test inhibitors targeting HRR pathways for cancer therapy, and study genetic variants linked to disease susceptibility.
Defects in XRCC2 are associated with Fanconi anemia-like phenotypes, increased cancer risk, and hypersensitivity to DNA crosslinking agents (e.g., cisplatin). Recombinant XRCC2 tools have advanced our understanding of how its dysfunction compromises replication fork restart and repair fidelity. Additionally, these proteins aid in developing biomarkers for HRR-deficient tumors and optimizing CRISPR-Cas9 gene-editing protocols, where precise HRR activity influences editing outcomes. Ongoing research focuses on its structural dynamics and therapeutic targeting to improve treatments for cancers reliant on alternative repair pathways.
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