首页 / 产品 / 蛋白 / 信号通路蛋白

Recombinant Human XAF1 protein

  • 中文名: XIAP相关因子1(XAF1)重组蛋白
  • 别    名: XAF1;BIRC4BP;XIAP-associated factor 1
货号: PA1000-3496
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点XAF1
Uniprot NoQ6GPH4
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-125aa
氨基酸序列MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEGD FSVCRNCKRH VVSANFTLHE AYCLRFLVLC PECEEPVPKE TMEEHCKLEH QQVGCTMCQQ SMQKSSLEFH KANECQERPV ECKFCKLDMQ LSKLELHESY CGSRTELCQG CGQFIMHRML A
预测分子量19 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于XAF1重组蛋白的3篇参考文献及其摘要内容概要:

---

1. **文献名称**:*XAF1 recombinant protein enhances TRAIL-induced apoptosis by regulating XIAP in human colon cancer cells*

**作者**:Lee MG, et al.

**摘要**:该研究通过表达并纯化XAF1重组蛋白,发现其能通过拮抗XIAP的凋亡抑制作用,显著增强TRAIL(肿瘤坏死因子相关凋亡诱导配体)在结肠癌细胞中的促凋亡效果,提示XAF1可能作为化疗增敏剂的应用潜力。

---

2. **文献名称**:*Expression, purification, and functional characterization of human XAF1 in E. coli*

**作者**:Wang Y, et al.

**摘要**:研究报道了利用大肠杆菌系统高效表达可溶性XAF1重组蛋白的优化方法,并通过体外实验证实重组XAF1可直接结合XIAP并抑制其抗凋亡活性,为后续结构及功能研究奠定基础。

---

3. **文献名称**:*XAF1 recombinant protein suppresses tumor growth via restoring p53 transcriptional activity in gastric cancer*

**作者**:Zhang L, et al.

**摘要**:研究发现,XAF1重组蛋白可通过激活p53信号通路抑制胃癌细胞增殖,并在小鼠模型中显著降低肿瘤负荷。机制研究表明,XAF1通过拮抗XIAP及调控p53靶基因实现协同抑癌作用。

---

**备注**:以上文献信息为基于领域内典型研究的模拟概括,实际引用时需核对具体来源及全文内容。建议通过PubMed或Web of Science以“XAF1 recombinant protein”为关键词检索最新文献。

背景信息

XAF1 (XIAP-associated factor 1) is a tumor suppressor protein first identified in 2000 as a binding partner of X-linked inhibitor of apoptosis protein (XIAP). Structurally, it contains seven zinc finger domains and a nuclear localization signal, enabling interactions with XIAP and other cellular components. Functionally, XAF1 acts as a pro-apoptotic factor by counteracting the anti-apoptotic activity of XIAP, a critical regulator of caspase enzymes in programmed cell death. Under normal conditions, XAF1 promotes caspase activation and sensitizes cells to apoptosis-inducing stimuli, including chemotherapeutic agents and radiation.

Its role in cancer has been extensively studied, as XAF1 expression is frequently downregulated in various malignancies (e.g., gastric, colorectal, and prostate cancers) due to promoter hypermethylation or epigenetic silencing. This loss correlates with tumor progression, chemotherapy resistance, and poor prognosis. Conversely, restoring XAF1 expression enhances cancer cell sensitivity to apoptosis, making it a potential therapeutic target.

Recombinant XAF1 protein, produced via bacterial or mammalian expression systems, retains biological activity for research and potential clinical applications. In vitro studies utilize the purified protein to investigate its molecular mechanisms, including XIAP neutralization, modulation of NF-κB signaling, and regulation of autophagy. Therapeutic strategies exploring XAF1 delivery via gene therapy or protein-based formulations aim to restore apoptotic pathways in resistant cancers. Current challenges include optimizing protein stability and delivery efficiency for in vivo applications. As research progresses, XAF1 recombinant protein continues to serve as a valuable tool for understanding apoptosis dysregulation in cancer and developing targeted therapies.

客户数据及评论

折叠内容

大包装询价

×