| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YAE1D1 |
| Uniprot No | Q9NRH1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-226aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSWVQAA SLIQGPGDKG DVFDEEADES LLAQREWQSN MQRRVKEGYR DGIDAGKAVT LQQGFNQGYK KGAEVILNYG RLRGTLSALL SWCHLHNNNS TLINKINNLL DAVGQCEEYV LKHLKSITPP SHVVDLLDSI EDMDLCHVVP AEKKIDEAKD ERLCENNAEF NKNCSKSHSG IDCSYVECCR TQEHAHSENP SPTWILEQTA SLVKQLGLSV DVLQHLKQL |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于YAE1D1重组蛋白的参考文献示例(注：内容为虚构，仅供格式参考)：
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1. **"Recombinant YAE1D1 Protein Expression and Structural Characterization"**
*Zhang, L. et al. (2021). Journal of Molecular Biology.*
摘要：本研究成功在大肠杆菌中表达并纯化YAE1D1重组蛋白，通过X射线晶体学解析其三维结构，发现其与EKC/KEOPS复合体的相互作用界面，为功能研究提供结构基础。
2. **"YAE1D1 in DNA Damage Response: Role in ATR Kinase Activation"**
*Smith, J.R. & Chen, H. (2019). Cell Reports.*
摘要：通过体外重组蛋白互作实验，揭示YAE1D1与ATR激酶的直接结合，证明其通过调控ATR信号通路参与DNA损伤修复，敲低YAE1D1导致细胞对辐射敏感性增加。
3. **"Functional Analysis of YAE1D1 in Ribosome Biogenesis"**
*Wang, Y. et al. (2020). Nucleic Acids Research.*
摘要：利用重组YAE1D1蛋白进行体外核糖体组装实验，发现其通过与18S rRNA结合调控核糖体小亚基成熟，为YAE1D1缺失导致发育缺陷提供机制解释。
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**提示**：以上为模拟示例，实际文献请通过PubMed、Web of Science等学术平台检索(关键词：YAE1D1 recombinant protein, EKC complex, DNA repair)。该基因可能关联KEOPS复合体，研究多集中于转录调控及基因组稳定性领域。
**Background of YAE1D1 Recombinant Protein**
YAE1D1 (Yae1 Domain-Containing 1) is a conserved eukaryotic protein implicated in mitochondrial function and cellular homeostasis. It belongs to the Yae1/Dph4 family, known for roles in post-translational modifications and iron-sulfur (Fe-S) cluster biogenesis, critical for enzyme activity and redox balance. Structurally, YAE1D1 contains a conserved Yae1-like domain, predicted to mediate protein-protein interactions, and a putative zinc-binding motif, suggesting involvement in metal ion coordination.
Studies link YAE1D1 to the mitochondrial *FAM210A-YBE1D1-YAE1D1* complex, which regulates mitochondrial protein synthesis and respiratory chain assembly. This complex interacts with mitochondrial ribosomes, influencing the translation of electron transport chain (ETC) components. Dysregulation of YAE1D1 disrupts ETC activity, leading to oxidative stress, impaired ATP production, and metabolic dysfunction.
YAE1D1 also intersects with cellular stress responses. It modulates pathways like mTOR signaling, affecting cell growth and autophagy under nutrient-deprived conditions. Additionally, its role in Fe-S cluster assembly connects it to genome stability, as Fe-S proteins are vital for DNA repair and replication.
Recombinant YAE1D1 protein is typically expressed in *E. coli* or mammalian systems, purified via affinity tags (e.g., His-tag), and utilized to study its molecular interactions, structural features, and functional mechanisms. Research focuses on its implications in mitochondrial diseases, cancer (where altered metabolism is a hallmark), and neurodegenerative disorders linked to mitochondrial failure.
Overall, YAE1D1 is a multifunctional player in mitochondrial biology and cellular stress adaptation, making it a potential therapeutic target for diseases rooted in metabolic and oxidative imbalances.
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