| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YIF1B |
| Uniprot No | Q5BJH7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMHPAGLAAAAAGTPRLRKWPSKRRIPV SQPGMADPHQLFDDTSSAQSRGYGAQRAPGGLSYPAASPTPHAAFLADPV SNMAMAYGSSLAAQGKELVDKNIDRFIPITKLKYYFAVDTMYVGRKLGLL FFPYLHQDWEVQYQQDTPVAPRFDVNAPD |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是我基于YIF1B相关研究领域构造的示例参考文献(注：以下文献为虚构示例，实际文献需通过PubMed等数据库检索)：
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1. **文献名称**: "Structural and Functional Characterization of Recombinant YIF1B Protein in Vesicular Trafficking"
**作者**: Li, X. et al.
**摘要**: 本研究通过大肠杆菌系统表达并纯化了重组YIF1B蛋白，结合X射线晶体学解析其三维结构，发现其通过C端结构域与Rab GTPases相互作用，调控内质网-高尔基体间的膜泡运输。功能实验表明，YIF1B敲低导致细胞分泌通路异常。
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2. **文献名称**: "YIF1B as a Novel Regulator of Cancer Cell Metastasis via EGFR Signaling"
**作者**: Wang, Y. et al.
**摘要**: 通过免疫共沉淀和质谱分析，发现YIF1B与EGFR形成复合物，促进其膜定位和下游信号激活。重组YIF1B蛋白的过表达增强乳腺癌细胞的迁移和侵袭能力，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**: "YIF1B Deficiency Disrupts Neuronal Protein Homeostasis and Induces Neurodegeneration in Mice"
**作者**: Suzuki, K. et al.
**摘要**: 构建YIF1B基因敲除小鼠模型，发现其海马区神经元内异常蛋白聚集和自噬缺陷。体外实验显示，重组YIF1B蛋白可挽救突触蛋白运输障碍，为神经退行性疾病机制提供新见解。
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4. **文献名称**: "Biochemical Analysis of YIF1B Interactions with COPII Coat Proteins"
**作者**: Garcia-Ruiz, I. et al.
**摘要**: 利用重组YIF1B蛋白进行体外结合实验，证实其与COPII复合物组分Sec23/Sec24直接互作，调控分泌蛋白的囊泡出芽过程。研究揭示了YIF1B在内膜系统质量控制中的关键作用。
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**建议**：实际文献检索可使用以下关键词在PubMed/Google Scholar中查找：
`YIF1B AND recombinant protein`、`YIF1B vesicular transport`、`YIF1B disease`。
YIF1B (Yip1 interacting factor homolog B) is a member of the Yip1 domain-containing protein family, which plays critical roles in intracellular membrane trafficking and vesicle transport. This conserved eukaryotic protein localizes primarily to the Golgi apparatus and endoplasmic reticulum (ER), where it facilitates cargo sorting and vesicular trafficking between organelles. Structurally, YIF1B contains multiple transmembrane domains and interacts with other trafficking regulators, such as Rab GTPases and SNARE proteins, to mediate membrane fusion and maintain organelle integrity.
Research indicates YIF1B is essential for neuronal development and synaptic function. It supports the anterograde transport of secretory vesicles carrying neurotransmitters and receptors, impacting synaptic plasticity. Dysregulation of YIF1B has been linked to neurodevelopmental disorders and neurodegenerative diseases, including Alzheimer's disease, where disrupted vesicular trafficking exacerbates pathological protein accumulation.
Recombinant YIF1B proteins are typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. These purified proteins enable in vitro investigations into their molecular interactions, structural features, and enzymatic activities. Epitope-tagged versions (e.g., His-tag, GFP-fusion) are widely used to track subcellular localization or protein-protein interactions via pull-down assays. Recent studies also explore YIF1B's potential role in cancer, as aberrant expression correlates with tumor metastasis and chemoresistance. Its involvement in secretory pathways makes it a candidate target for modulating cellular secretion processes in therapeutic contexts. However, detailed mechanistic insights into YIF1B's regulatory networks remain limited, necessitating further research to elucidate its physiological and pathological significance.
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