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产品描述：绵羊白介素10(IL-10)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是重要靶点，它是一种具有多种生物学活性的细胞因子。其研究机制主要在于抗炎方面，能抑制多种促炎因子的产生。在免疫调节中发挥关键作用，可调控免疫细胞功能和活性，目前在自身免疫病、炎症疾病等领域是研究热点。试剂盒检测范围为5 pg/mL-80 pg/mL，的应用场景包括：绵羊免疫系统功能研究、炎症性疾病机制探索、疫苗效力评估、感染模型的IL - 10动态监测、自身免疫疾病研究中的IL - 10动态监测、生物制剂开发中的IL - 10动态监测、畜牧兽医研究、比较免疫学实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：IL10
Uniprot No.：Q29408
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：5 pg/mL-80 pg/mL
灵敏度：5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 82-88 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 800.2020.1960.199 400.3650.3450.355 200.6230.6410.632 101.1471.1681.158 52.3442.1892.267 | ||||||
货期：3-5 working days
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