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产品描述：绵羊胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF1含量。IGF1即胰岛素样生长因子1，在细胞的生长、增殖和分化等过程中发挥关键作用。其异常表达与肿瘤发生、发展等密切相关，研究主要围绕其信号通路展开，旨在通过干预信号传导，为癌症等疾病治疗提供新靶点与思路。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于绵羊生长发育评估、饲料营养学研究、生长激素相关疾病模型建立等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写：IGF1
Uniprot No.：P10763
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 87-101 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of sheep IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-92 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.8422.7952.8192.657 5002.0152.0762.0461.884 2501.1371.1611.1490.987 1250.5880.6040.5960.434 62.50.3570.3720.3650.203 31.20.2780.2930.2860.124 15.60.2340.2420.2380.076 00.1640.1600.162 | ||||||
货期：3-5 working days
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