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产品描述：牛缪勒管抑制物质/抗缪勒管激素(AMH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AMH含量。抗缪勒管激素（AMH）由卵巢颗粒细胞分泌，可反映卵巢储备功能，与生殖健康密切相关。研究机制上，AMH参与卵泡发育调控，抑制始基卵泡启动及窦前卵泡对FSH的敏感性。临床常检测其水平评估卵巢功能，辅助生殖领域应用广泛。试剂盒检测范围为0.5 ng/mL-200 ng/mL，适用于畜牧业中繁殖能力研究、卵巢储备功能评估、生殖相关疾病模型构建等科研场景，组织匀浆检测功能还可拓展至生殖器官局部AMH表达分析。为繁殖生物学、发育生物学等领域的科研工作者提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
缩写：AMH
Uniprot No.：P03972
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.5 ng/mL-200 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 93-105 | |||||
回收率：
| The recovery of bovine AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average2000.1340.1390.13737.50.2110.2030.2076.250.4950.4850.4901.750.9680.9870.9780.51.1461.1541.15002.2262.2502.238 | ||||||
货期：3-5 working days
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