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产品描述：绵羊脂联素(ADIPOQ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的ADIPOQ含量。ADIPOQ即脂联素，由脂肪组织分泌。它在能量代谢、胰岛素敏感性等方面发挥重要作用。研究机制上，脂联素通过与受体结合激活下游信号通路，参与调节糖脂代谢、抗炎、抗动脉粥样硬化等，对代谢性疾病防治有潜在价值。试剂盒检测范围为6.25 ng/mL-100 ng/mL，该产品适用于绵羊代谢调控机制研究、疾病模型建立及营养干预实验等科研领域，尤其在探索肥胖、糖尿病相关动物模型中具有重要应用价值，为畜牧业健康监测和基础医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ADIPOQ
种属：Ovis aries (Sheep)
样本类型：serum, plasma
检测范围：6.25 ng/mL-100 ng/mL
灵敏度：3.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-106 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of sheep ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-103 | ||||
| EDTA plasma (n=4) | 89 | 84-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 1000.0900.0870.089 500.2350.2550.245 250.5660.5800.573 12.50.9260.9850.956 6.251.6271.5621.595 02.3852.3182.352 | ||||||
货期：3-5 working days
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