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产品描述：牛神经肽Y(NPY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NPY含量。NPY即神经肽Y，是一种广泛分布于中枢和外周神经系统的肽类神经递质。它在调节摄食行为、能量代谢、心血管活动等方面发挥重要作用。研究机制上，主要通过与相应受体结合，激活细胞内信号转导途径，进而产生一系列生理效应。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可用于科研领域探索NPY在牛的能量代谢调控、应激生理响应、神经内分泌网络中的作用机制，例如在饲喂干预、环境压力模型或神经功能研究中动态监测NPY的表达水平变化，为畜牧学、动物生理学及比较医学研究提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NPY ELISA Kit; Pro-neuropeptide Y [Cleaved into: Neuropeptide Y ELISA Kit; Neuropeptide tyrosine ELISA Kit; NPY); C-flanking peptide of NPY ELISA Kit; CPON)] ELISA Kit
缩写：NPY
Uniprot No.：Q6RUW3
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-93 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of bovine NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 91 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6042.4902.5472.444102.1132.2212.1672.06451.6571.7111.6841.5812.51.1141.0561.0850.9821.250.7120.6870.7000.5970.6250.4130.4280.4210.3180.3120.2780.2600.2690.16600.1050.1010.103 | ||||||
货期：3-5 working days
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