关键信息
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基因名
ISG15 E1/UBE1L
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简介
The ISG15 E1/UBE1L protein initiates ubiquitination by adenylating the C-terminal glycine of ubiquitin with ATP to form a thioester bond with its cysteine residue. This produces ubiquitin-E1 thioester and free AMP. ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is the recombinant human-derived ISG15 E1/UBE1L protein, expressed by Sf9 insect cells , with N-8*His, N-Strep labeled tag.
- 应用
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别名
Ubiquitin-like modifier-activating enzyme 7; UBA7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog; UBE1L; UBE2
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种属
Human
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表达系统
Baculovirus
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标签
N-StrepⅡ;N-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P41226
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表达区间
D2-L1012
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蛋白长度
Partial
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分子量
114.7 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ISG15 (Interferon-stimulated gene 15) is a ubiquitin-like protein that plays a crucial role in the immune response, particularly in the regulation of antiviral and inflammatory processes. The E1 enzyme UBE1L (ubiquitin-activating enzyme 1-like) is responsible for the initial step in the ISG15 conjugation pathway, activating ISG15 for subsequent transfer to target proteins, a process known as ISGylation. This post-translational modification has been implicated in various biological functions, including protein stability, localization, and activity modulation, thereby influencing cellular responses to stress and pathogen invasion. Research has shown that ISGylation can enhance the antiviral capabilities of host cells and modulate the immune system's response, making it a key area of interest in the study of host-pathogen interactions and immune regulation. The exploration of ISG15 and UBE1L, particularly through recombinant protein studies, provides valuable insights into their functional mechanisms and their potential as therapeutic targets in diseases characterized by dysregulated immune responses, such as cancer and autoimmune disorders. Understanding the biochemistry and biological roles of both ISG15 and UBE1L can pave the way for novel strategies in the development of antiviral therapies and immunomodulatory treatments.
