| 纯度 | >90%SDS-PAGE. |
| 种属 | Rat |
| 靶点 | Igg-2a |
| Uniprot No | P20760 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-322aa |
| 氨基酸序列 | AETTAPSVYP LAPGTALKSN SMVTLGCLVK GYFPEPVTVT WNSGALSSGV HTFPAVLQSG LYTLTSSVTV PSSTWSSQAV TCNVAHPASS TKVDKKIVPR ECNPCGCTGS EVSSVFIFPP KTKDVLTITL TPKVTCVVVD ISQNDPEVRF SWFIDDVEVH TAQTHAPEKQ SNSTLRSVSE LPIVHRDWLN GKTFKCKVNS GAFPAPIEKS ISKPEGTPRG PQVYTMAPPK EEMTQSQVSI TCMVKGFYPP DIYTEWKMNG QPQENYKNTP PTMDTDGSYF LYSKLNVKKE TWQQGNTFTC SVLHEGLHNH HTEKSLSHSP GK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IgG2a重组蛋白的虚构参考文献示例，供参考：
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1. **标题**: *High-Yield Production and Functional Characterization of Recombinant IgG2a in Mammalian Expression Systems*
**作者**: Smith A, et al.
**摘要**: 本研究利用CHO细胞系统表达并纯化重组小鼠IgG2a抗体，优化了载体设计和培养条件以提高产量。通过ELISA和表面等离子体共振(SPR)验证了其对抗原的特异性结合，并证实其Fc区域可有效激活补体途径，为大规模生产功能性IgG2a提供了方案。
2. **标题**: *Comparative Analysis of IgG Subclass Effector Functions: Role of Recombinant IgG2a in FcγR Binding*
**作者**: Johnson R, et al.
**摘要**: 通过对比IgG1、IgG2a和IgG2b亚型的重组蛋白，发现IgG2a对激活型Fcγ受体(如FcγRIV)具有高亲和力，显著增强巨噬细胞介导的吞噬作用。研究强调了IgG2a在抗肿瘤抗体疗法中的潜在优势。
3. **标题**: *Recombinant IgG2a Engineered for Enhanced Antibody-Dependent Cellular Cytotoxicity (ADCC) in Cancer Models*
**作者**: Brown K, et al.
**摘要**: 通过对IgG2a的Fc区域进行点突变(如S239D/I332E)，显著提高了其与NK细胞表面FcγRIIIa的结合能力。体内实验表明，工程化IgG2a在黑色素瘤模型中诱导更强的ADCC效应，抑制肿瘤生长。
4. **标题**: *Application of Recombinant IgG2a in Autoimmune Disease Intervention*
**作者**: Lee M, et al.
**摘要**: 利用重组IgG2a抗体靶向自身抗原(如dsDNA)，在系统性红斑狼疮(SLE)小鼠模型中观察到炎症标志物减少和肾脏损伤缓解。研究提示IgG2a的免疫调节功能可能依赖其独特的糖基化模式。
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**注**：以上文献为模拟示例，实际引用需检索PubMed、Google Scholar等数据库获取真实文献。
**Background of IgG2a Recombinant Protein**
Immunoglobulin G subclass 2a (IgG2a) is a critical antibody isotype in murine immune responses, playing a central role in adaptive immunity. As one of the four IgG subclasses in mice (IgG1. IgG2a, IgG2b, IgG3), IgG2a is distinguished by its heavy chain structure, particularly in the Fc region, which dictates effector functions such as complement activation and binding to Fc gamma receptors (FcγRs) on immune cells. This subclass is strongly associated with pro-inflammatory responses, enhancing pathogen clearance through antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP).
Recombinant IgG2a proteins are engineered using biotechnological platforms, often expressed in mammalian cell systems (e.g., CHO or HEK293 cells) to ensure proper glycosylation and structural fidelity. These proteins retain the native heterotetrameric structure—two heavy chains and two light chains linked by disulfide bonds—while allowing customization of variable regions for antigen specificity. Recombinant technology enables large-scale production with high purity and batch-to-batch consistency, addressing limitations of traditional hybridoma-derived antibodies.
In research, recombinant IgG2a is widely utilized to study immune mechanisms, particularly Th1-biased responses, and to develop therapeutic monoclonal antibodies (mAbs). Its potent Fc-mediated effector functions make it valuable in oncology (e.g., targeting tumor antigens) and infectious disease models. Additionally, IgG2a is employed as a control in immunoassays, flow cytometry, and in vivo studies to mimic or modulate immune activity.
Compared to IgG1. IgG2a exhibits stronger binding to activating FcγRs (e.g., FcγRIV in mice), driving robust effector cell engagement. This property is leveraged in therapeutic antibody design, where Fc engineering can fine-tune effector functions for specific applications. However, IgG2a’s pro-inflammatory nature may also pose risks in certain clinical contexts, necessitating careful consideration during development.
Overall, recombinant IgG2a proteins serve as indispensable tools in immunology and biotechnology, bridging foundational research and translational drug development.
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