| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNHIT3 |
| Uniprot No | Q15649 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-155aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMASLKCS TVVCVICLEK PKYRCPACRV PYCSVVCFRK HKEQCNPETR PVEKKIRSAL PTKTVKPVEN KDDDDSIADF LNSDEEEDRV SLQNLKNLGE SATLRSLLLN PHLRQLMVNL DQGEDKAKLM RAYMQEPLFV EFADCCLGIV EPSQNEES |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ZNHIT3重组蛋白的模拟参考文献(非真实文献，仅供示例格式参考)：
1. **文献名称**: "ZNHIT3 regulates chromatin remodeling and DNA damage response through its interaction with SRCAP complex"
**作者**: Li X, Wang Q, et al. (2021)
**摘要**: 研究利用重组ZNHIT3蛋白进行体外结合实验，发现其作为染色质重塑复合体SRCAP的关键组分，通过调控组蛋白H2A.Z的替换参与DNA损伤修复通路。
2. **文献名称**: "Functional characterization of ZNHIT3 in hepatocellular carcinoma progression via recombinant protein-based assays"
**作者**: Zhang Y, Chen L, et al. (2020)
**摘要**: 通过重组ZNHIT3蛋白的过表达和敲低实验，证明其在肝癌细胞中通过调控Wnt/β-catenin信号通路抑制肿瘤迁移和侵袭，提示其作为潜在抑癌基因的作用。
3. **文献名称**: "Structural insights into ZNHIT3-PICH interaction during mitotic chromosome condensation"
**作者**: Gupta R, Lee S, et al. (2019)
**摘要**: 利用重组ZNHIT3蛋白进行冷冻电镜结构解析，揭示其与PICH蛋白结合形成复合体，调控有丝分裂中染色体凝聚的动态过程。
4. **文献名称**: "ZNHIT3 deficiency disrupts ribosome biogenesis via impaired 18S rRNA processing"
**作者**: Müller M, Schmidt A, et al. (2018)
**摘要**: 通过重组ZNHIT3蛋白的体外核糖体组装实验，发现其与UTP-C亚基相互作用，影响18S rRNA加工，导致核糖体生成异常及细胞增殖受阻。
(注：以上文献为模拟内容，实际研究中请通过PubMed/Google Scholar检索真实文献。)
ZNHIT3 (Zinc Finger HIT-Type Containing 3) is a member of the zinc finger HIT-type protein family, characterized by a conserved HIT (HIS1-like transcriptional repressor) domain and zinc finger motifs. This nuclear protein plays a role in chromatin remodeling, transcriptional regulation, and DNA damage repair. It has been identified as a component of protein complexes like SHREC (Snf2/Hdac-containing Repressor Complex) and TORC (Target of Rapamycin Complex), linking it to epigenetic silencing, heterochromatin maintenance, and cellular stress responses. Structurally, ZNHIT3 contains an N-terminal HIT domain, which may mediate hydrolytic enzyme activity, and a C-terminal zinc finger domain critical for protein-DNA or protein-protein interactions.
Recombinant ZNHIT3 protein is engineered for functional studies, typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems with affinity tags (e.g., His-tag, GST-tag) to facilitate purification. Its recombinant form enables investigations into molecular mechanisms, such as interactions with histone deacetylases (HDACs), methyltransferases, or chromatin-associated proteins. Research highlights its involvement in cell cycle regulation, genomic stability, and developmental processes, with dysregulation implicated in cancers and neurological disorders. Current applications include in vitro binding assays, enzymatic activity analyses, and disease modeling, providing insights for potential therapeutic targeting in chromatin-related pathologies.
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