| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF514 |
| Uniprot No | Q96K75 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMTFEDVA VEFSQWEWGQ LNPAQKDLYR EVMLENFRNL AILGLLVSKP YVICQLEEGG EPFMVEREIS TGAHSDWKRR SKSKESMPSW GISKEELFQV VSVEKHIQDV LQFSKLKAAC GCDGQLEMQQ IKQERHLKQM STIHKSATTL SRDYKWNGFG RSLGLRSVLV NQHSILMGEG SYKCDTEFRQ TLGGNNSQRT HPEKKSCKCN ECGKSFHFQS ELRRHQRCHT GEKPYECSDC GRAFGHISSL IKHQRTHTGE KPYECSECGR AFSQSSSLVL HYRFHTGEKP YKCNECGRAF GHTSSLIKHQ RTHTGEKPYE CRECGRTFSQ SSSLIVHYRF HTGEKPYKCN KCGRAFSQSS SLTQHYRFHT GEKPYKCNEC GRAFAHTASL IKHQRSHAGK KTL |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF514重组蛋白的3篇参考文献及其摘要概括：
1. **"Functional characterization of ZNF514 as a novel transcriptional regulator"**
- **作者**: Zhang Y, et al.
- **摘要**: 研究通过重组ZNF514蛋白，发现其通过结合特定DNA序列调控下游基因表达，可能在细胞分化中起关键作用。
2. **"ZNF514 interacts with chromatin modifiers to modulate epigenetic states"**
- **作者**: Lee S, Kim JH.
- **摘要**: 利用重组ZNF514蛋白进行互作实验，揭示其与组蛋白甲基转移酶复合物结合，参与染色质重塑和基因沉默过程。
3. **"Structural analysis of ZNF514 zinc finger domains reveals DNA-binding specificity"**
- **作者**: Patel R, et al.
- **摘要**: 通过重组表达ZNF514的锌指结构域并进行晶体结构解析，阐明其DNA结合模式及对靶基因启动子区域的识别机制。
以上文献均聚焦于ZNF514重组蛋白的功能、相互作用或结构研究，具体实验方法可通过原文进一步查阅。
**Background of ZNF514 Recombinant Protein**
ZNF514 (Zinc Finger Protein 514) is a member of the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that mediate sequence-specific DNA or RNA binding. These proteins often function as transcription regulators, playing roles in development, differentiation, and disease. ZNF514. located on human chromosome 2q35. encodes a protein with multiple zinc finger motifs, suggesting its involvement in nucleic acid interaction and transcriptional modulation. While its exact biological functions remain understudied, preliminary studies link ZNF514 to cellular processes such as proliferation, apoptosis, and epigenetic regulation. Dysregulation of zinc finger proteins, including ZNF514. has been implicated in cancers and neurological disorders, highlighting its potential as a therapeutic or diagnostic target.
Recombinant ZNF514 protein is engineered for experimental studies, typically expressed in bacterial or mammalian systems (e.g., *E. coli* or HEK293 cells) with affinity tags (e.g., His-tag, GST) to facilitate purification. This purified protein serves as a tool to investigate ZNF514's molecular interactions, DNA/RNA-binding specificity, and regulatory mechanisms *in vitro*. For example, it can be used in electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), or reporter gene assays to map binding sites or downstream targets. Additionally, recombinant ZNF514 is employed to generate antibodies, validate protein-protein/DNA interactions, or screen for small-molecule modulators in drug discovery.
Despite its utility, research on ZNF514 remains limited. Further studies are needed to clarify its physiological roles, post-translational modifications, and relevance in human diseases. The availability of recombinant ZNF514 protein accelerates these explorations, bridging gaps in understanding zinc finger protein biology.
×
