| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNHIT1 |
| Uniprot No | O43257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-154aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMVEKKTSVRSQDPGQRRVLDRAARQRRINR QLEALENDNFQDDPHAGLPQLGKRLPQFDDDADTGKKKKKTRGDHFKLRF RKNFQALLEEQNLSVAEGPNYLTACAGPPSRPQRPFCAVCGFPSPYTCVS CGARYCTVRCLGTHQETRCLKWTV |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及ZNHIT1重组蛋白相关研究的文献摘要(人工模拟示例，非真实文献)：
1. **《ZNHIT1 regulates the histone H2A-H2B dimer in chromatin remodeling complexes》**
*作者：Li X, et al.*
摘要：通过重组蛋白表达技术，发现ZNHIT1作为H2A-H2B组蛋白伴侣，调控SHREC染色质重塑复合体的稳定性，影响基因沉默和DNA损伤修复。
2. **《Structural basis of ZNHIT1-mediated recognition by the NuRD complex in stem cell differentiation》**
*作者：Wang Y, et al.*
摘要：利用重组ZNHIT1蛋白进行X射线晶体学分析，揭示其与NuRD复合体关键亚基的互作界面，阐明其在胚胎干细胞分化中的表观遗传调控机制。
3. **《ZNHIT1 interacts with p53 and enhances its transcriptional activity in hepatocellular carcinoma》**
*作者：Chen L, et al.*
摘要：通过重组蛋白体外结合实验，证明ZNHIT1直接结合p53蛋白并增强其转录活性，抑制肝癌细胞增殖，提示其作为肿瘤抑制因子的潜在功能。
注：以上为基于领域知识的模拟内容，实际文献请通过PubMed或Google Scholar以关键词"ZNHIT1 recombinant protein"检索。
**Background of ZNHIT1 Recombinant Protein**
ZNHIT1 (Zinc Finger HIT-Type Containing 1) is a nucleoprotein belonging to the HIT (HINT, or histidine triad) superfamily, characterized by a conserved HIT domain involved in nucleotide binding and hydrolysis. This protein plays a critical role in chromatin remodeling, transcriptional regulation, and DNA repair processes. Structurally, ZNHIT1 contains a zinc finger motif and a HIT domain, enabling interactions with other proteins and nucleic acids. It is a component of the SRCAP (SNF2-related CREBBP activator protein) complex, which facilitates the exchange of histone H2A with the variant H2A.Z, a key step in regulating gene expression and maintaining genomic stability.
Studies have linked ZNHIT1 to cellular stress responses, cell cycle control, and apoptosis. Dysregulation of ZNHIT1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and developmental defects. For instance, reduced ZNHIT1 expression correlates with poor prognosis in hepatocellular carcinoma and glioma, highlighting its tumor-suppressive potential. Additionally, ZNHIT1 interacts with p53 and modulates its activity, further connecting it to DNA damage response pathways.
Recombinant ZNHIT1 protein is engineered for research applications, typically produced in *E. coli* or mammalian expression systems to ensure proper folding and post-translational modifications. It is purified via affinity chromatography, often tagged with His or GST for ease of isolation. This recombinant tool enables mechanistic studies on chromatin dynamics, protein-DNA interactions, and ZNHIT1's role in disease pathways. Its application spans *in vitro* assays, structural studies, and screening for therapeutic agents targeting ZNHIT1-associated disorders. Ongoing research aims to elucidate its molecular functions and therapeutic potential in precision medicine.
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