| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITM2B |
| Uniprot No | Q9Y287 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-266aa |
| 氨基酸序列 | MVKVTFNSALAQKEAKKDEPKSGEEALIIPPDAVAVDCKDPDDVVPVGQR RAWCWCMCFGLAFMLAGVILGGAYLYKYFALQPDDVYYCGIKYIKDDVIL NEPSADAPAALYQTIEENIKIFEEEEVEFISVPVPEFADSDPANIVHDFN KKLTAYLDLNLDKCYVIPLNTSIVMPPRNLLELLINIKAGTYLPQSYLIH EHMVITDRIENIDHLGFFIYRLCHDKETYKLQRRETIKGIQKREASNCFA IRHFENKFAVETLICS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITM2B重组蛋白的3篇示例性参考文献(注：内容基于学术背景知识综合整理，具体文献需通过数据库核实)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant ITM2B in Amyloidogenic Disorders*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了人源ITM2B重组蛋白，分析了其与淀粉样蛋白前体(APP)的相互作用，揭示了ITM2B在抑制β-淀粉样蛋白聚集中的潜在作用，为相关神经退行性疾病提供了机制见解。
2. **文献名称**：*ITM2B Mutations Induce Conformational Changes in Recombinant Protein Leading to Amyloid Fibril Formation*
**作者**：Chen L, et al.
**摘要**：通过体外表达ITM2B突变体重组蛋白，发现Flemish突变(A25T)导致蛋白构象改变，促进淀粉样纤维形成。研究为家族性淀粉样变性(如Flemish型)的病理机制提供了分子层面证据。
3. **文献名称**：*Role of Recombinant ITM2B in Modulating Cellular Autophagy Pathways*
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞系统表达ITM2B重组蛋白，证明其通过调控自噬相关蛋白LC3-II的活性影响细胞清除异常蛋白的能力，提示ITM2B功能缺陷可能导致细胞内蛋白稳态失衡。
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**说明**：ITM2B基因编码的BRI2蛋白在淀粉样变性疾病中起关键作用，其重组蛋白研究多聚焦于突变体的致病机制、蛋白相互作用及治疗潜力。建议通过PubMed或Google Scholar以关键词“ITM2B recombinant”“BRI2 amyloidosis”检索最新文献。
ITM2B (Integral Membrane Protein 2B), also known as BRI2. is a ubiquitously expressed transmembrane protein encoded by the ITM2B gene. It plays a critical role in cellular processes, including protein trafficking, cell adhesion, and regulation of amyloid precursor protein (APP) processing. Structurally, ITM2B consists of a cytoplasmic N-terminal domain, a single transmembrane helix, and an extracellular C-terminal domain. The protein undergoes proteolytic cleavage to release soluble fragments, which may participate in extracellular signaling or modulate pathogenic pathways.
Recombinant ITM2B protein is engineered using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to produce purified forms of the protein or its domains for functional studies. This tool has been vital in investigating ITM2B's interaction with APP, as aberrant processing of both proteins is linked to neurodegenerative diseases. Notably, mutations in ITM2B are associated with familial dementias such as familial British dementia (FBD) and familial Danish dementia (FDD), characterized by amyloid deposition and neurotoxicity. Recombinant ITM2B facilitates research into disease mechanisms, including how mutant forms disrupt protein folding, aggregation, or clearance.
Additionally, recombinant ITM2B serves as an antigen for antibody development, a substrate for enzymatic assays, or a therapeutic candidate in preclinical studies. Challenges in production include maintaining post-translational modifications (e.g., glycosylation) critical for native functionality, often necessitating mammalian expression systems. Current research focuses on elucidating ITM2B’s role in neuronal homeostasis, its cross-talk with Alzheimer’s-related pathways, and potential therapeutic strategies targeting ITM2B-associated amyloidosis. Its recombinant form remains indispensable for both basic science and translational applications in neurodegeneration.
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