Recombinant Human CALCB protein

中文名： 降钙素基因相关肽2(CALCB)重组蛋白
别名： CALCB；CALC2；Calcitonin gene-related peptide 2

货号: PA1000-3605

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	CALCB
Uniprot No	P10092
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-118aa
氨基酸序列	MGFRKFSPFL ALSILVLYQA GSLQAAPFRS ALESSPDPAT LSKEDARLLL AALVQDYVQM KASELKQEQE TQGSSSAAQK RACNTATCVT HRLAGLLSRS GGMVKSNFVP TNVGSKAF
预测分子量	36 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CALCB(降钙素基因相关肽β)重组蛋白的3篇参考文献示例：

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1. **标题**：High-yield Expression and Purification of Recombinant Human CALCB Protein for Neurobiological Studies

**作者**：Zhang L, Chen H, Wang Y

**摘要**：本研究利用大肠杆菌表达系统成功表达重组人CALCB蛋白，并通过镍柱亲和层析纯化获得高纯度产物。体外实验证实该蛋白可激活神经元cAMP信号通路，促进背根神经节细胞轴突生长，为神经再生研究提供了工具。

2. **标题**：Structural Characterization of Recombinant CALCB and Its Receptor Binding Mechanism

**作者**：Tanaka K, Müller CE

**摘要**：通过圆二色谱和表面等离子体共振技术解析了重组CALCB的二级结构，并发现其C端区域是结合CGRP受体的关键位点，为靶向该受体的药物开发提供了结构依据。

3. **标题**：Therapeutic Potential of Recombinant CALCB in Chronic Migraine: A Preclinical Study

**作者**：Russo AF, Goadsby PJ

**摘要**：在慢性偏头痛大鼠模型中，鞘内注射重组CALCB显著抑制硬脑膜血管扩张和c-Fos蛋白表达，效果持续48小时以上，提示其作为新型偏头痛治疗生物制剂的潜力。

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注：以上文献为示例，实际引用时需核实真实存在的论文。建议通过PubMed、Web of Science等数据库，以关键词“recombinant CALCB protein”“CGRP beta expression”等检索最新研究。

背景信息

CALCB (Calcitonin Gene-Related Peptide Beta), also known as β-CGRP, is a neuropeptide encoded by the CALCB gene, a paralog of the CALCA gene that produces α-CGRP. Both peptides share structural similarities, including a conserved N-terminal disulfide bridge and C-terminal amidation, but differ in specific amino acid residues and tissue distribution. CALCB is primarily expressed in the peripheral and central nervous systems, as well as in the gastrointestinal tract, and functions as a potent vasodilator, neurotransmitter, and modulator of inflammatory responses. It binds to the CGRP receptor, a G protein-coupled receptor complex (CLR/RAMP1), triggering cAMP signaling pathways involved in pain transmission, vascular tone regulation, and neurogenic inflammation.

Recombinant CALCB protein is produced using biotechnological platforms such as bacterial (E. coli) or mammalian expression systems, often with affinity tags (e.g., His-tag) for purification. Its production enables precise study of CGRP receptor interactions, particularly in differentiating β-CGRP-specific roles from the more extensively studied α-CGRP. While α-CGRP is linked to migraines and cardiovascular homeostasis, CALCB’s distinct functions—such as potential roles in gut motility, immune modulation, and tissue repair—are emerging research focuses. Recombinant CALCB also serves as a critical tool in drug discovery, especially for therapies targeting CGRP pathways, including migraine treatments (e.g., monoclonal antibodies, gepants). However, challenges remain in characterizing isoform-specific effects due to receptor overlap. Current studies emphasize its therapeutic potential in inflammatory diseases, wound healing, and metabolic disorders, highlighting the need for further mechanistic exploration.