Human soluble transferrin receptor,sTfR ELISA Kit

大包装询价

货号: IPD-E09100h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人可溶性转铁蛋白受体(sTfR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的sTfR含量。本产品可广泛应用于基础医学研究中铁代谢机制探索、营养性缺铁模型评估、血液疾病相关分子通路分析，以及药物或膳食干预对铁吸收影响的体外实验数据获取，为科研人员提供稳定可靠的定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CD 71 ELISA Kit; CD71 ELISA Kit; CD71 antigen ELISA Kit; IMD46 ELISA Kit; OTTHUMP00000208523 ELISA Kit; OTTHUMP00000208524 ELISA Kit; OTTHUMP00000208525 ELISA Kit; p90 ELISA Kit; sTfR ELISA Kit; T9 ELISA Kit; TFR 1 ELISA Kit; TfR ELISA Kit; TfR1 ELISA Kit; TFR1_HUMAN ELISA Kit; TFRC ELISA Kit; TR ELISA Kit; Transferrin receptor (p90 CD71) ELISA Kit; Transferrin receptor protein 1; serum form ELISA Kit; Trfr ELISA Kit
缩写：
sTfR
Uniprot No.：
P02786
种属：
Homo sapiens (Human)
样本类型：
serum, plasma
检测范围：
Request Information
灵敏度：
Request Information
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
3600	2.578	2.634	2.606	2.606
1800	1.563	1.667	1.615	1.615
900	0.834	0.828	0.831	0.831
450	0.441	0.429	0.435	0.435
150	0.192	0.201	0.197	0.197
0	0.078	0.083	0.081	0.081

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human sTfR antibody.
Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated sTfR antibody (6 ml/bottle) ---Bind to the C-Peptide in the samples or standards and react with the substrate to make the solution chromogenic.
Substrate A (1 x 7 ml)
Substrate B (1 x 7 ml)
Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.