首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人粒细胞巨噬细胞集落刺激因子（GM-CSF)抗体(酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清样本中的GM-CSF Ab含量。GM-CSF抗体是一种针对粒细胞-巨噬细胞集落刺激因子（GM-CSF）的抗体，GM-CSF是一种重要的免疫调节因子，参与免疫细胞增殖和分化。GM-CSF抗体通过阻断GM-CSF与其受体的结合，抑制GM-CSF的活性，从而调节免疫反应，用于治疗某些炎症和自身免疫性疾病。研究机制主要涉及GM-CSF信号通路和免疫调节过程。试剂盒检测范围为0.625 ng/mL-40 ng/mL，科研人员可将其应用于探索GM-CSF在疾病发生发展中的生物学机制、评估药物干预对细胞因子网络的影响，或作为免疫相关动物模型研究的配套检测工具，为免疫学、肿瘤学及炎症性疾病领域的基础研究提供有效的实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GM-CSF Ab
种属：Homo sapiens (Human)
样本类型：serum
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-101 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 84-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-106 | |||||
回收率：
| The recovery of human GM-CSF antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 86-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5972.6482.6232.518 202.1232.1312.1272.022 101.4661.4721.4691.364 50.8310.8960.8640.759 2.50.4720.4940.4830.378 1.250.3120.2970.3050.200 0.6250.2250.2410.2330.128 00.1020.1070.105 | ||||||
货期：3-5 working days
×
