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产品描述：鱼白介素1β(IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B是重要的炎症细胞因子，在多种炎症性疾病及免疫反应中起关键作用。研究机制上，它参与激活免疫细胞、调节炎症信号通路。围绕IL1B的研究聚焦于其在疾病发生发展中的作用，为开发抗炎、免疫调节药物提供靶点。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本产品广泛应用于水产动物免疫学研究、鱼类病原感染模型构建、疫苗效力评估以及水产养殖环境胁迫对机体免疫功能影响等科研场景，为深入解析鱼类先天免疫机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：il1bInterleukin-1 beta ELISA Kit
缩写：IL-1β
Uniprot No.：Q9YGD3
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 82-89 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 90-99 | ||||||
| 1:4 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 94-106 | ||||||
回收率：
| The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-102 | |||||
| EDTA plasma (n=4) | 89 | 82-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected2002.9892.7782.8842.7761002.4672.4322.4502.342501.7741.8661.8201.712250.9560.9320.9440.83612.50.4360.4560.4460.3386.250.2580.2700.2640.1563.120.1560.1550.1560.04800.1050.1110.108 | |||||||
货期：3-5 working days
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