首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒

Dog angiotensin 1-7 (Ang1-7) ELISA kit

  • 中文名: 犬血管紧张素1-7(Ang1-7)ELISA kit
货号: IPD-EQ027505DO
Price: ¥3000
数量:
大包装询价

产品详情

  • 产品描述:犬血管紧张素1-7(Ang1-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、组织培养上清液样本中的Ang1-7含量。Ang1-7是重要靶点。它属于肾素-血管紧张素系统成员。其研究机制主要体现在可通过结合Mas受体,发挥舒张血管、降低血压、抑制炎症和纤维化等作用,在心血管疾病、肾脏疾病等研究领域有潜在治疗价值。试剂盒检测范围为6.25 pg/mL-400 pg/mL,研究者可将其应用于犬类心血管疾病模型、肾脏功能研究、代谢综合征机制探索,以及药物干预或基因调控对Ang1 - 7通路影响的体外实验分析,为深入解析RAS系统在病理生理过程中的作用提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 缩写:Ang1-7

  • 种属:Canis lupus familiaris (Dog) (Canis familiaris)

  • 样本类型:serum, plasma, tissue homogenates, cell culture supernates

  • 检测范围:6.25 pg/mL-400 pg/mL

  • 灵敏度:1.56 pg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Cardiovascular

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of dog Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)

    1:1Average %87

    Range %83-92

    1:2Average %100

    Range %97-103

    1:4Average %86

    Range %83-89

    1:8Average %98

    Range %94-102

  • 回收率:

    The recovery of dog Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)8581-89

    EDTA plasma (n=4)9185-94

















  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected4003.0612.9002.9812.8712002.5412.5682.5552.4451001.7511.7681.7601.650501.0271.1001.0640.954250.5050.5490.5270.41712.50.3010.2940.2980.1886.250.1980.1890.1940.08400.1120.1080.110 







  • 货期:3-5 working days

客户数据及评论

折叠内容

大包装询价

×