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产品描述:犬血管紧张素1-7(Ang1-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、组织培养上清液样本中的Ang1-7含量。Ang1-7是重要靶点。它属于肾素-血管紧张素系统成员。其研究机制主要体现在可通过结合Mas受体,发挥舒张血管、降低血压、抑制炎症和纤维化等作用,在心血管疾病、肾脏疾病等研究领域有潜在治疗价值。试剂盒检测范围为6.25 pg/mL-400 pg/mL,研究者可将其应用于犬类心血管疾病模型、肾脏功能研究、代谢综合征机制探索,以及药物干预或基因调控对Ang1 - 7通路影响的体外实验分析,为深入解析RAS系统在病理生理过程中的作用提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:Ang1-7
种属:Canis lupus familiaris (Dog) (Canis familiaris)
样本类型:serum, plasma, tissue homogenates, cell culture supernates
检测范围:6.25 pg/mL-400 pg/mL
灵敏度:1.56 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of dog Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 87 | |||||
Range % | 83-92 | ||||||
1:2 | Average % | 100 | |||||
Range % | 97-103 | ||||||
1:4 | Average % | 86 | |||||
Range % | 83-89 | ||||||
1:8 | Average % | 98 | |||||
Range % | 94-102 |
回收率:
The recovery of dog Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 85 | 81-89 | |||||
EDTA plasma (n=4) | 91 | 85-94 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected4003.0612.9002.9812.8712002.5412.5682.5552.4451001.7511.7681.7601.650501.0271.1001.0640.954250.5050.5490.5270.41712.50.3010.2940.2980.1886.250.1980.1890.1940.08400.1120.1080.110 |
货期:3-5 working days
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