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产品描述：鱼多巴胺(DA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DA含量。DA即多巴胺，是脑内重要神经递质，在运动控制、认知、情感及内分泌调节等过程中发挥关键作用。研究机制方面，围绕其合成、释放、与受体结合及代谢等环节展开，探究其在帕金森病、精神分裂症等疾病中异常变化以寻找治疗靶点。试剂盒检测范围为62.5 pg/mL-1000 pg/mL，该产品适用于鱼类神经生物学研究、水产养殖健康监测、环境污染物对水生生物神经系统的毒性评估等科研领域，特别在水体环境毒理学实验中，可通过检测鱼类血液或组织DA水平评估重金属、农药等污染物对中枢神经系统的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DA
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-1000 pg/mL
灵敏度：62.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.1540.1550.155 5000.2780.2720.275 2500.5030.5180.511 1251.1351.1741.155 62.52.3452.4282.387 | ||||||
货期：3-5 working days
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