首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠甲状旁腺激素(PTH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆样本中的PTH含量。PTH（甲状旁腺激素）是一种调节血钙水平的典型内分泌激素，对维持钙磷代谢和骨骼健康至关重要。其通过结合骨细胞和肾脏细胞中的受体PTH1R，激活下游信号通路，调节钙磷代谢。近年来，PTH及其相关肽（PTHrPs）在骨质疏松症治疗等领域展现出显著疗效，成为研究热点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于基础研究中的钙代谢调控机制探索、甲状旁腺功能相关疾病模型建立、药物干预效果评估等方向，特别针对骨代谢研究、肾性骨营养不良模型分析及内分泌系统相关实验提供可靠检测工具，为科研人员解析PTH在生理病理过程中的作用机制提供标准化解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PTH
种属：Mus musculus (Mouse)
样本类型：serum, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 87-99 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 96-104 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 84-94 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 89-100 | ||||||
回收率：
| The recovery of mouse PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 84-95 | |||||
| EDTA plasma (n=4) | 104 | 97-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4002.6702.5842.6272.5072001.9971.9371.9671.8471001.3601.3391.3501.230500.9310.9000.9160.796250.5140.5010.5080.38812.50.3060.2980.3020.1826.250.2240.2210.2230.10300.1220.1180.120 | |||||||
货期：3-5 working days
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