首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抗环胍氨酸肽抗体(Anti-CCP-antibody)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Anti-CCP-Ab含量。Anti - CCP - Ab即抗环瓜氨酸肽抗体，它是类风湿关节炎高度特异的血清学标志物。其研究机制主要是能识别环化修饰的抗原决定簇，与类风湿关节炎发病机制中的自身免疫反应相关，检测它有助于类风湿关节炎的早期诊断及病情评估。试剂盒检测范围为6.25 U/mL-400 U/mL，适用于基础科研中疾病机制探索、药物研发阶段的药效学评价以及动物模型中的生物标志物动态监测等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Anti-CCP-Ab
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：6.25 U/mL-400 U/mL
灵敏度：1.56 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-106 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 89-104 | |||||
回收率：
| The recovery of mouse anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-110 | ||||
| EDTA plasma (n=4) | 95 | 83-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| U/mlOD1OD2AverageCorrected 4002.6972.7412.7192.573 2002.2012.2242.2132.067 1001.5071.4831.4951.349 500.8340.8490.8420.696 250.4920.5110.5020.356 12.50.2970.3100.3040.158 6.250.2260.2380.2320.086 00.1480.1440.146 | ||||||
货期：3-5 working days
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