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产品描述：仓鼠C反应蛋白(CRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CRP含量。CRP（C反应蛋白）是一种急性时相反应蛋白，在机体发生感染、炎症、组织损伤时，血浆中CRP水平会急剧升高。其研究机制主要集中在参与炎症反应调节，可激活补体系统、调节免疫细胞功能等，常作为炎症和疾病活动性的重要监测指标。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于仓鼠模型中的炎症机制研究、药物干预效果评价或疾病相关生物标志物探索等科研场景，为实验动物模型的炎症状态监测提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CRP ELISA kit; PTX1C-reactive protein ELISA kit
缩写：CRP
Uniprot No.：P49262
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:10 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 94-104 | |||||
回收率：
| The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-93 | ||||
| EDTA plasma (n=4) | 96 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.0172.0752.0461.8884001.5921.5841.5881.4302001.0621.0361.0490.8911000.6250.5860.6060.448500.4560.4410.4490.291250.3090.2930.3010.14312.50.2310.2180.2250.06700.1550.1610.158 | ||||||
货期：3-5 working days
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