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产品描述:人趋化因子配体17(CXCL17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的CXCL17含量。CXCL17是一种趋化因子,在免疫调节等生理过程中发挥作用。目前研究聚焦于其在肿瘤微环境里对免疫细胞的趋化,影响肿瘤免疫逃逸,还探索其与炎症性疾病的关联,想通过揭示机制为开发肿瘤及炎症治疗新策略提供依据。试剂盒检测范围为125 pg/mL-8000 pg/mL,可广泛应用于细胞因子网络研究、炎症相关疾病机制探索、肿瘤免疫微环境分析等科研领域,为体外实验中的CXCL17表达水平检测提供标准化工具,支持药物开发或病理机制研究中的生物标志物分析需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:CXCL17 ELISA Kit; VCC1 ELISA Kit; UNQ473/PRO842C-X-C motif chemokine 17 ELISA Kit; 6-Cys CXCL17 ELISA Kit; Dendritic cell and monocyte chemokine-like protein ELISA Kit; DMC ELISA Kit; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17] ELISA Kit
缩写:CXCL17
Uniprot No.:Q6UXB2
种属:Homo sapiens (Human)
样本类型:serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围:125 pg/mL-8000 pg/mL
灵敏度:31.25 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CXCL17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 82-94 | |||||
1:2 | Average % | 90 | ||||
Range % | 84-95 | |||||
1:4 | Average % | 96 | ||||
Range % | 92-100 | |||||
1:8 | Average % | 93 | ||||
Range % | 90-96 |
回收率:
The recovery of human CXCL17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 90-102 | ||||
EDTA plasma (n=4) | 98 | 90-104 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected80002.1742.1992.1872.02240001.4591.4991.4791.31420000.9990.9730.9860.82110000.6380.6920.6650.5005000.4500.4680.4590.2942500.3240.3420.3330.1681250.2470.2590.2530.08800.1640.1660.165 |
货期:3-5 working days
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