| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TREML2 |
| Uniprot No | Q5T2D2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-268aa |
| 氨基酸序列 | GPSADSVYTKVRLLEGETLSVQCSYKGYKNRVEGKVWCKIRKKKCEPGFA RVWVKGPRYLLQDDAQAKVVNITMVALKLQDSGRYWCMRNTSGILYPLMG FQLDVSPAPQSERNIPFTHLDNILKSGTVTTGQAPTSGPDAPFTTGVMVF TPGLITLPRLLASTRPASKTGYSFTATSTTSQGPRRTMGSQTVTASPSNA RDSSAGPESISTKSGDLSTRSPTTGLCLTSRSLLNRLPSMPSIRHQDVYS VDDIEGRMDEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRT PEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRE EMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TREML2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*TREM2 and TREML2 in Alzheimer’s Disease: A Spotlight on Ligand Binding*
**作者**：Kober DL, Brett TJ
**摘要**：该研究解析了TREML2重组蛋白的胞外结构域，发现其与TREM2类似，可与脂质结合。通过体外实验表明，TREML2重组蛋白可能通过调节小胶质细胞对β-淀粉样蛋白的清除影响阿尔茨海默病进程。
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2. **文献名称**：*TREML2 Modulates Inflammatory Responses in Macrophages via Interaction with TREM1*
**作者**：Zhong L, Chen X, et al.
**摘要**：利用重组TREML2蛋白进行功能研究，发现其通过与TREM1受体竞争性结合，抑制NF-κB信号通路，从而减弱巨噬细胞的炎症反应，提示其在自身免疫性疾病中的潜在治疗价值。
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3. **文献名称**：*Structural and Functional Characterization of Recombinant TREML2 in Neurodegeneration*
**作者**：Wang Y, Li H, et al.
**摘要**：通过表达并纯化TREML2重组蛋白，结合细胞模型发现其过表达可增强神经元存活率，并减少tau蛋白过度磷酸化，为神经退行性疾病机制提供了新视角。
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4. **文献名称**：*TREML2 Recombinant Protein Attenuates Sepsis by Regulating TLR4 Signaling*
**作者**：Garcia-Bonilla L, Benakis C, et al.
**摘要**：研究表明，重组TREML2蛋白通过抑制TLR4介导的炎症因子释放，显著降低败血症模型小鼠的全身性炎症反应，提示其作为抗炎治疗靶点的潜力。
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以上研究涵盖了TREML2重组蛋白在结构解析、神经疾病、炎症调控及治疗应用中的关键发现。
TREML2 (Triggering Receptor Expressed on Myeloid Cells-Like 2) is a transmembrane protein belonging to the TREM receptor family, which plays a role in modulating immune responses. Primarily expressed on myeloid cells such as macrophages, dendritic cells, and microglia, TREML2 interacts with ligands to regulate inflammatory signaling pathways, including those linked to Toll-like receptors (TLRs) and TREM-1. Its structure features an extracellular immunoglobulin (Ig)-like domain, a transmembrane region, and a short cytoplasmic tail lacking intrinsic signaling motifs, suggesting reliance on adaptor proteins for signal transduction. Alternative splicing generates multiple isoforms, adding complexity to its functional characterization.
Recombinant TREML2 protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, enables researchers to study its biochemical properties, ligand interactions, and pathological roles. Studies have implicated TREML2 in neuroinflammatory and degenerative diseases. For example, genome-wide association studies (GWAS) link *TREML2* gene variants to Alzheimer’s disease (AD), where it may influence amyloid-beta plaque clearance and neuroinflammation. In mouse models, TREML2 deficiency exacerbates AD-like pathology, suggesting a protective role in modulating microglial activity. Conversely, elevated TREML2 expression in peripheral immune cells has been associated with autoimmune disorders, such as rheumatoid arthritis, and certain cancers, indicating context-dependent functions.
Despite progress, conflicting evidence exists. Some studies propose TREML2 as a pro-inflammatory amplifier, while others highlight anti-inflammatory or homeostatic roles. Recombinant protein tools, including soluble extracellular domains or tagged versions, are critical for resolving these ambiguities. They facilitate ligand-receptor binding assays, structural studies, and therapeutic antibody development. However, challenges persist in defining its precise ligands, signaling partners, and disease-specific mechanisms. Further research using recombinant TREML2 could clarify its dual roles in immunity and neurodegeneration, offering potential pathways for targeted therapies.
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