| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPON2 |
| Uniprot No | Q9BUD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-331aa |
| 氨基酸序列 | QPLG GESICSARAL AKYSITFTGK WSQTAFPKQY PLFRPPAQWS SLLGAAHSSD YSMWRKNQYV SNGLRDFAER GEAWALMKEI EAAGEALQSV HEVFSAPAVP SGTGQTSAEL EVQRRHSLVS FVVRIVPSPD WFVGVDSLDL CDGDRWREQA ALDLYPYDAG TDSGFTFSSP NFATIPQDTV TEITSSSPSH PANSFYYPRL KALPPIARVT LVRLRQSPRA FIPPAPVLPS RDNEIVDSAS VPETPLDCEV SLWSSWGLCG GHCGRLGTKS RTRYVRVQPA NNGSPCPELE EEAECVPDNC V |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPON2重组蛋白的参考文献示例(注：以下内容为示例性虚构，实际文献请通过学术数据库查询)：
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1. **文献名称**: *Recombinant SPON2 Protein Inhibits Tumor Metastasis by Modulating Integrin Signaling*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组SPON2蛋白，并发现其能够通过抑制整合素β1/FAK信号通路，显著降低结直肠癌细胞的迁移和侵袭能力，提示其在抗肿瘤治疗中的潜在应用价值。
2. **文献名称**: *SPON2 Recombinant Protein Attenuates Inflammatory Response in Macrophages via TLR4/NF-κB Pathway*
**作者**: Kim S, et al.
**摘要**: 该研究利用哺乳动物细胞表达纯化的SPON2重组蛋白，发现其可通过结合TLR4受体并抑制NF-κB的活化，减少巨噬细胞中促炎因子(如TNF-α、IL-6)的释放，为炎症性疾病治疗提供新方向。
3. **文献名称**: *Expression and Functional Characterization of Recombinant SPON2 in Neuronal Regeneration*
**作者**: Müller R, et al.
**摘要**: 通过昆虫杆状病毒系统表达SPON2重组蛋白，证实其能够促进神经元轴突生长，并通过激活MAPK/ERK通路增强损伤后神经元的再生能力，为神经修复研究提供实验依据。
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**建议**：如需真实文献，请访问PubMed或Google Scholar，搜索关键词“recombinant SPON2 protein”、“SPON2 expression”或结合具体研究领域(如癌症、炎症)筛选。
**Background of SPON2 Recombinant Protein**
SPON2 (Spondin-2), also known as Mindin, is an extracellular matrix protein belonging to the spondin family, characterized by its conserved thrombospondin type-1 repeat (TSR) domains. It plays critical roles in immune regulation, neural development, and inflammatory responses by mediating cell-matrix and cell-cell interactions. SPON2 is secreted by various cell types, including macrophages, endothelial cells, and neurons, and functions as a pattern recognition molecule that binds pathogens (e.g., bacteria) to enhance innate immune responses. It also modulates integrin signaling, promoting cell adhesion, migration, and angiogenesis.
In the nervous system, SPON2 supports axon guidance and synaptic plasticity by interacting with neuronal receptors. Its involvement in cancer progression has drawn significant attention, as SPON2 is often overexpressed in tumors (e.g., colorectal, prostate, and liver cancers), where it promotes metastasis, drug resistance, and immune evasion.
Recombinant SPON2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, bioactive forms for research and therapeutic applications. It retains functional domains, enabling studies on its role in inflammatory diseases, neural repair, and cancer biology. Researchers utilize SPON2 recombinant protein to investigate signaling pathways, screen drug candidates, or develop diagnostic tools. Its dual role as an immune modulator and oncogenic factor highlights its therapeutic potential, though context-dependent functions necessitate careful exploration. Overall, SPON2 recombinant protein serves as a vital tool for deciphering its multifaceted roles in health and disease.
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