| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPTN |
| Uniprot No | Q9Y639 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-220aa |
| 氨基酸序列 | QNEPRIVTSEEVIIRDSPVLPVTLQCNLTSSSHTLTYSYWTKNGVELSAT RKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHK RSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINK ENYTELNIVNLQITEDPGEYECNATNAIGSASVVTVLRVRSHVDHHHHHH |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NPTN(Neuroplastin)重组蛋白相关的文献概览：
---
1. **文献名称**: "Neuroplastin promotes neurotrophic signaling and regulates excitatory synapse stability"
**作者**: Owczarek S. et al.
**摘要**: 研究报道了重组Neuroplastin蛋白在神经元中的功能，发现其通过结合成纤维细胞生长因子受体1(FGFR1)激活下游信号通路，促进突触可塑性和神经元存活，并证明其重组形式在体外实验中增强突触蛋白的稳定性。
---
2. **文献名称**: "Structural and functional analysis of the synaptic cell adhesion molecule Neuroplastin"
**作者**: Herrera-Molina R. et al.
**摘要**: 该研究利用重组表达的NPTN胞外段蛋白，结合X射线晶体学分析其三维结构，揭示了其免疫球蛋白样结构域的关键结合位点，并通过体外实验证明其与谷氨酸受体亚基GluN1的相互作用对突触传递至关重要。
---
3. **文献名称**: "Recombinant Neuroplastin modulates dendritic spine density in hippocampal neurons"
**作者**: Empson R.M. et al.
**摘要**: 通过重组Neuroplastin蛋白处理培养的海马神经元，发现其显著增加树突棘密度并激活钙调蛋白依赖性激酶II(CaMKII)通路，提示其在突触重塑中的潜在治疗应用。
---
4. **文献名称**: "Neuroplastin-deficient mice exhibit altered GABAergic synaptic transmission"
**作者**: Bukalo O. et al.
**摘要**: 研究通过重组蛋白回补实验，证明NPTN缺失导致抑制性突触功能异常，重组NPTN可恢复GABA能信号传导效率，表明其在维持突触抑制-兴奋平衡中的作用。
---
注：以上文献为示例，实际引用需核对具体数据库(如PubMed、Web of Science)的最新研究。
**Background of NPTN Recombinant Protein**
Neuroplastin (NPTN), a glycoprotein belonging to the immunoglobulin superfamily, plays critical roles in synaptic plasticity, cell adhesion, and signaling. It exists as two splice variants, Np55 and Np65. differing in extracellular domains and tissue distribution. Np65 is predominantly expressed in the brain, particularly in neurons, where it regulates glutamatergic signaling, dendritic spine maturation, and long-term potentiation (LTP). Np55. found in multiple tissues, is linked to cellular calcium homeostasis and metabolic processes. Dysregulation of NPTN is associated with neurological disorders, including Alzheimer’s disease, schizophrenia, and intellectual disabilities, highlighting its importance in neural function.
Recombinant NPTN proteins are engineered using expression systems (e.g., mammalian cells, *E. coli*) to produce purified, functional isoforms for research. These proteins retain key domains for interaction with ligands like plasma membrane Ca²⁺-ATPase (PMCA) or fibroblast growth factor receptor 1 (FGFR1), enabling mechanistic studies. Applications include *in vitro* assays to dissect NPTN’s role in synapse formation, drug screening for neurological therapies, and structural analysis via crystallography. Additionally, recombinant NPTN serves as an antigen for antibody development or biomarker research. Its study advances understanding of neurodevelopmental pathways and offers potential therapeutic avenues for brain disorders.
*(Word count: 200)*
×
