| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAI3 |
| Uniprot No | O60242 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-880aa |
| 氨基酸序列 | AQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFS KKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVFLQYD KNFIQIRRVFPTNFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTW LESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLILLNNVVLPLNE QTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKR VPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGT HCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTP PQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGTQQRS RQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGG WERRIRTCQGAVITGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVW KRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRH LQHSIKEHLAKGQRMLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILR NVTDTFKRASYIPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIEL MQVIEDFIHIVGMGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKG RKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNLDLIL PTLRNYTVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDD SKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESS GTPSVTVDHHHHHH |
| 预测分子量 | 98 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于BAI3重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
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1. **"Structural characterization of recombinant BAI3 ectodomain and its role in neuronal adhesion"**
*Authors: Smith J, Lee H, et al.*
*摘要：通过X射线晶体学解析BAI3重组蛋白胞外域结构，揭示其与细胞黏附相关的构象特征，并验证其在神经元突触形成中的功能。*
2. **"BAI3 interacts with ELMO1/DOCK180 to regulate phagocytosis in microglia"**
*Authors: Chen L, Wang Y, et al.*
*摘要：利用重组BAI3蛋白进行免疫共沉淀实验，发现其与ELMO1/DOCK180复合物相互作用，调控小胶质细胞吞噬功能及下游RhoG信号通路。*
3. **"Recombinant BAI3 protein alleviates angiogenesis in a mouse tumor model"**
*Authors: Tanaka K, Rodriguez M, et al.*
*摘要：在小鼠模型中注射重组BAI3蛋白，证明其通过抑制VEGFR2磷酸化减少肿瘤血管生成，为抗肿瘤治疗提供潜在策略。*
4. **"CRISPR/Cas9-mediated BAI3 knockout combined with recombinant protein rescue reveals its role in synaptic plasticity"**
*Authors: Gupta R, Park S, et al.*
*摘要：通过基因编辑结合重组BAI3蛋白回补实验，证实BAI3缺失导致海马神经元突触可塑性异常，且重组蛋白可部分恢复长时程增强(LTP)功能。*
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如需真实文献，建议通过PubMed或Google Scholar检索关键词"BAI3 recombinant protein"或"BAI1-3 adhesion GPCR"。
**Background of BAI3 Recombinant Protein**
BAI3 (Brain-Specific Angiogenesis Inhibitor 3), also known as ADGRB3. is a member of the adhesion G protein-coupled receptor (adhesion-GPCR) subfamily. It is primarily expressed in the central nervous system (CNS) and plays critical roles in neuronal development, synaptic plasticity, and cell-cell communication. Structurally, BAI3 contains a large extracellular N-terminal region with thrombospondin type-1 repeats (TSP1), which mediate interactions with extracellular matrix components and cell-surface proteins. Its intracellular domain interacts with signaling molecules, enabling it to regulate pathways involved in cell adhesion, migration, and apoptosis.
BAI3 gained attention for its role in regulating excitatory synapse formation through binding to presynaptic PCDH7 (protocadherin-7) and postsynaptic neuroligin-1. This interaction highlights its importance in maintaining synaptic stability and neural network function. Additionally, BAI3 is implicated in pathological conditions, including neurodevelopmental disorders, neurodegenerative diseases, and cancers. For example, aberrant BAI3 expression has been linked to glioblastoma progression, where it modulates tumor cell invasion and angiogenesis.
Recombinant BAI3 protein is engineered for *in vitro* and *in vivo* studies to dissect its molecular mechanisms. Produced using mammalian or insect expression systems, it retains post-translational modifications essential for ligand-binding and signaling activities. Researchers utilize BAI3 recombinant protein to investigate its therapeutic potential, such as targeting synaptic dysfunction in autism or blocking tumor metastasis. Its versatility in structural and functional studies makes it a valuable tool for exploring adhesion-GPCR biology and developing precision therapies.
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