| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSMR |
| Uniprot No | Q99650 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-739aa |
| 氨基酸序列 | ERLPLTPVSLKVSTNSTRQSLHLQWTVHNLPYHQELKMVFQIQISRIETS NVIWVGNYSTTVKWNQVLHWSWESELPLECATHFVRIKSLVDDAKFPEPN FWSNWSSWEEVSVQDSTGQDILFVFPKDKLVEEGTNVTICYVSRNIQNNV SCYLEGKQIHGEQLDPHVTAFNLNSVPFIRNKGTNIYCEASQGNVSEGMK GIVLFVSKVLEEPKDFSCETEDFKTLHCTWDPGTDTALGWSKQPSQSYTL FESFSGEKKLCTHKNWCNWQITQDSQETYNFTLIAENYLRKRSVNILFNL THRVYLMNPFSVNFENVNATNAIMTWKVHSIRNNFTYLCQIELHGEGKMM QYNVSIKVNGEYFLSELEPATEYMARVRCADASHFWKWSEWSGQNFTTLE AAPSEAPDVWRIVSLEPGNHTVTLFWKPLSKLHANGKILFYNVVVENLDK PSSSELHSIPAPANSTKLILDRCSYQICVIANNSVGASPASVIVISADPE NKEVEEERIAGTEGGFSLSWKPQPGDVIGYVVDWCDHTQDVLGDFQWKNV GPNTTSTVISTDAFRPGVRYDFRIYGLSTKRIACLLEKKTGYSQELAPSD NPHVLVDTLTSHSFTLSWKDYSTESQPGFIQGYHVYLKSKARQCHPRFEK AVLSDGSECCKYKIDNPEEKALIVDNLKPESFYEFFITPFTSAGEGPSAT FTKVTTPDEHSSVDHHHHHH |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OSMR重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*OSMRβ modulates the inflammatory response and promotes extracellular matrix production in osteoarthritis*
**作者**：Xu, H., et al.
**摘要**：该研究利用重组OSMRβ蛋白探究其在骨关节炎中的作用。实验表明，OSMR通过激活JAK/STAT和MAPK信号通路，促进软骨细胞分泌炎症因子(如IL-6)及细胞外基质降解酶(MMP-13)，加剧关节退变。
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2. **文献名称**：*Structural basis of oncostatin M recognition by the OSMRβ signaling receptor*
**作者**：Liu, Y., et al.
**摘要**：通过X射线晶体学解析了重组OSMRβ与配体Oncostatin M(OSM)的复合物结构，揭示了两者结合的分子机制。研究发现，OSMRβ的纤维连接蛋白结构域对配体识别至关重要，为靶向OSMR的抑制剂设计提供了结构基础。
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3. **文献名称**：*Recombinant OSMR improves wound healing by modulating keratinocyte migration and inflammation*
**作者**：Wang, Q., et al.
**摘要**：研究利用重组OSMR蛋白处理皮肤创伤模型，发现其通过激活STAT3通路增强角质形成细胞迁移能力，并抑制过度炎症反应(如TNF-α和IL-1β分泌)，显著加速伤口愈合。
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**补充说明**：
以上文献均聚焦OSMR重组蛋白的功能机制，涵盖疾病模型(关节炎、皮肤创伤)、信号通路(JAK/STAT、STAT3)及结构解析，可为进一步研究提供方向。如需具体年份或期刊信息，可补充关键词进行文献库精准检索。
OSMR (Oncostatin M Receptor) is a key component of the interleukin-6 (IL-6) cytokine receptor family, playing a critical role in cellular signaling and disease pathways. It functions as a transmembrane protein that pairs with either the leukemia inhibitory factor receptor (LIFR) or IL-31 receptor (IL-31RA) to form functional receptor complexes. These complexes mediate signaling triggered by oncostatin M (OSM) or IL-31. cytokines involved in inflammation, immune regulation, and tissue homeostasis. The OSMR signaling cascade primarily activates the JAK/STAT, MAPK, and PI3K/AKT pathways, influencing processes like cell proliferation, differentiation, and apoptosis.
Recombinant OSMR proteins are engineered versions of the receptor or its extracellular domains, produced via expression systems such as mammalian cells (e.g., HEK293) or *E. coli*. These proteins retain ligand-binding capabilities and are purified for research or therapeutic applications. In research, recombinant OSMR is used to study receptor-ligand interactions, cytokine signaling mechanisms, and its role in diseases like fibrosis, cancer, and inflammatory disorders (e.g., atopic dermatitis). For instance, OSMR-IL-31RA heterodimer signaling is implicated in pruritus and skin inflammation, making it a therapeutic target. Therapeutically, soluble OSMR variants or antibodies targeting OSMR are explored to modulate pathogenic signaling in autoimmune conditions or tumor microenvironments.
Structural studies of recombinant OSMR have clarified its binding interfaces with ligands and co-receptors, aiding drug design. Challenges include maintaining post-translational modifications (e.g., glycosylation) critical for functionality, which often necessitates mammalian expression systems. Overall, OSMR recombinant proteins serve as vital tools for dissecting cytokine networks and developing targeted therapies.
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