| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCAM1 |
| Uniprot No | P13591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 611-710aa |
| 氨基酸序列 | EPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVRYRALSSEWKPEI RLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIP |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCAM1重组蛋白的3篇参考文献及其简要摘要：
1. **"Production of recombinant human NCAM in HEK293 cells and analysis of its glycosylation pattern"**
- **作者**: Kiselyov, V.V. et al.
- **摘要**: 该研究在HEK293细胞中表达了重组人NCAM1蛋白，分析了其复杂的糖基化修饰，并验证了糖基化对NCAM1介导的细胞间黏附功能的关键作用。
2. **"NCAM1-FGFR interaction promotes neurite outgrowth via the lipid kinase PIP5Kγ"**
- **作者**: Ditlevsen, D.K. et al.
- **摘要**: 通过重组NCAM1胞外域蛋白，揭示了NCAM1与成纤维细胞生长因子受体(FGFR)的结合机制，及其通过激活PIP5Kγ信号通路促进神经突生长的功能。
3. **"Recombinant NCAM fragments as potential therapeutic agents for blood-brain barrier penetration"**
- **作者**: Herve, J.C. et al.
- **摘要**: 研究利用重组NCAM1的特定功能结构域(如Ig1-2模块)，开发能够穿透血脑屏障的靶向药物递送载体，验证了其在小鼠模型中的有效性。
4. **"Soluble NCAM as a biomarker in cancer: Development of an ELISA using recombinant NCAM"**
- **作者**: Sørensen, A.L. et al.
- **摘要**: 通过重组NCAM1蛋白建立了一种高灵敏度ELISA检测方法，用于量化癌症患者血清中的可溶性NCAM水平，证明其作为肿瘤转移标志物的潜力。
以上文献涵盖了重组NCAM1的表达优化、结构功能研究、治疗应用开发及临床诊断工具设计等多个方向。
Neural Cell Adhesion Molecule 1 (NCAM1), also known as CD56. is a transmembrane glycoprotein belonging to the immunoglobulin superfamily of cell adhesion molecules (IgCAMs). It plays a pivotal role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and neural development. NCAM1 mediates homophilic (NCAM-NCAM) and heterophilic interactions with extracellular matrix components or other cell surface receptors, influencing intracellular signaling pathways involved in cell migration, survival, and differentiation. Its structure includes five immunoglobulin-like domains, two fibronectin type III repeats, and a cytoplasmic domain that interacts with cytoskeletal proteins and kinases.
Recombinant NCAM1 proteins are engineered in vitro using expression systems such as mammalian cells, insect cells, or prokaryotes to study its biological functions or therapeutic potential. These proteins often retain key functional domains and post-translational modifications (e.g., polysialylation) critical for activity. Researchers utilize recombinant NCAM1 to investigate mechanisms underlying neural regeneration, cancer metastasis (where NCAM1 expression is frequently dysregulated), and neurodevelopmental disorders. It also serves as a tool to explore ligand-receptor interactions or to develop diagnostic/therapeutic agents targeting NCAM1-associated pathways. Studies have highlighted its dual roles in tumor progression—either suppressing or promoting metastasis depending on context—and its involvement in neurodegenerative diseases like Alzheimer’s. Recombinant NCAM1 thus bridges basic research and translational applications, offering insights into cell adhesion biology and opportunities for drug discovery.
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