| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BTN3A3 |
| Uniprot No | O00478 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-248aa |
| 氨基酸序列 | Q FSVLGPSGPI LAMVGEDADL PCHLFPTMSA ETMELRWVSS SLRQVVNVYA DGKEVEDRQS APYRGRTSIL RDGITAGKAA LRIHNVTASD SGKYLCYFQD GDFYEKALVE LKVAALGSDL HIEVKGYEDG GIHLECRSTG WYPQPQIKWS DTKGENIPAV EAPVVADGVG LYAVAASVIM RGSSGGGVSC IIRNSLLGLE KTASISIADP FFRSAQPW |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BTN3A3重组蛋白的虚构参考文献示例(注：内容为模拟创作，非真实文献)：
1. **文献名称**：Structural and Functional Analysis of BTN3A3 Recombinant Protein in Immune Modulation
**作者**：Smith J, et al.
**摘要**：本研究通过重组表达BTN3A3蛋白，解析其晶体结构，揭示其与γδ T细胞受体相互作用的机制，证实BTN3A3在调控先天免疫应答中的关键作用。
2. **文献名称**：BTN3A3 as an Antiviral Factor: Recombinant Protein Expression and Activity Validation
**作者**：Zhang L, et al.
**摘要**：报道BTN3A3重组蛋白在体外抑制多种RNA病毒复制的功能，发现其通过激活干扰素通路增强宿主抗病毒免疫，为抗病毒治疗提供新靶点。
3. **文献名称**：Epigenetic Regulation of BTN3A3 in Cancer and Its Recombinant Protein Application
**作者**：Jones R, et al.
**摘要**：探讨BTN3A3在肿瘤中的表观遗传沉默现象，并通过重组蛋白实验证明其过表达可抑制癌细胞增殖，提示其作为肿瘤免疫治疗潜力分子。
4. **文献名称**：BTN3A3 Recombinant Protein Mediates Phosphoantigen Sensing in Vγ9Vδ2 T Cells
**作者**：Braesch C, et al.
**摘要**：利用重组BTN3A3蛋白验证其与细胞内磷抗原结合的能力，阐明其通过AP1信号通路激活Vγ9Vδ2 T细胞抗感染的分子机制。
(注：以上文献为示例性质，实际研究需查阅真实数据库。)
BTN3A3 (Butyrophilin Subfamily 3 Member A3) is a transmembrane protein belonging to the butyrophilin family, which shares structural similarities with immunoglobulin (Ig) superfamily members. Primarily expressed in immune cells and certain epithelial tissues, BTN3A3 is part of a protein network involved in modulating immune responses, particularly γδ T cell-mediated immunity. Its extracellular region contains two Ig-like domains (V and C1), while the intracellular domain carries a B30.2/PRY-SPRY motif, suggesting roles in protein-protein interactions and immune signaling.
Research highlights BTN3A3's critical function in phosphoantigen sensing, a process enabling Vγ9Vδ2 T cells to detect metabolic changes in infected or cancerous cells. BTN3A3 cooperates with BTN3A1 to bind endogenous phosphorylated metabolites (e.g., ATP metabolites), triggering γδ T cell activation against pathogens or malignancies. This mechanism positions BTN3A3 as a potential therapeutic target for infections (e.g., Mycobacterium tuberculosis) and cancer immunotherapy.
Recombinant BTN3A3 protein, typically produced in mammalian or insect cell systems to preserve post-translational modifications, enables structural and functional studies. Researchers use it to map antigen-binding interfaces, characterize interaction partners, and develop antibody-based therapies. Recent studies also explore its role in autoimmune regulation and viral immune evasion, with some viruses potentially hijacking BTN3A3 pathways to suppress host defenses.
Despite progress, BTN3A3's precise signaling mechanisms remain partially elusive. Its recombinant form continues to be instrumental in resolving its immunomodulatory duality – balancing pro-inflammatory responses while maintaining immune tolerance – making it a compelling subject for both basic immunology and translational drug discovery.
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