| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK1G2 |
| Uniprot No | P78368 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-415aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSKAGGGRSSHGIRSSGTSSGVLMVGPNFR VGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLS ATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAI QLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYID PETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLR GSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRL DFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPS QPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADET KCCCFFKRRKRKSLQRHK |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSNK1G2重组蛋白的虚构参考文献示例(仅供示例用途)：
1. **"Recombinant expression and functional characterization of human CSNK1G2 in cancer cell signaling"**
- **作者**: Zhang, L. et al.
- **摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组CSNK1G2蛋白，验证其激酶活性及在Wnt/β-catenin信号通路中的调控作用，发现其过表达促进肿瘤细胞增殖。
2. **"Structural insights into CSNK1G2 kinase domain and its interaction with small molecule inhibitors"**
- **作者**: Thompson, R. & Singh, M.
- **摘要**: 通过X射线晶体学解析了重组CSNK1G2激酶结构域的三维结构，揭示了其与ATP类似物及抑制剂的结合模式，为靶向药物设计提供依据。
3. **"High-throughput screening identifies novel CSNK1G2 inhibitors with anti-leukemic activity"**
- **作者**: Gomez, S. et al.
- **摘要**: 利用重组CSNK1G2蛋白建立高通量筛选平台，鉴定出两种新型抑制剂，可显著抑制白血病细胞生长并诱导凋亡。
4. **"Role of recombinant CSNK1G2 in circadian rhythm regulation: In vitro phosphorylation assays"**
- **作者**: Hirota, T. & Kay, S.
- **摘要**: 研究重组CSNK1G2对核心生物钟蛋白PER2的磷酸化作用，证实其在哺乳动物昼夜节律调控中的关键功能。
(注：以上文献为模拟内容，实际文献需通过PubMed/Google Scholar等平台检索。)
CSNK1G2. also known as casein kinase 1 gamma 2 (CK1γ2), is a serine/threonine protein kinase belonging to the CK1 family. It plays a regulatory role in multiple cellular processes, including Wnt/β-catenin signaling, cell cycle progression, and circadian rhythms. As a member of the CK1γ subfamily, CSNK1G2 shares structural homology with other isoforms (CK1γ1 and CK1γ3) but exhibits distinct tissue-specific expression patterns and substrate preferences. The kinase is characterized by a conserved catalytic domain and unique C-terminal regions that influence its subcellular localization and interaction partners.
Recombinant CSNK1G2 protein is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) to study its enzymatic activity and biological functions. Researchers utilize purified recombinant protein for in vitro kinase assays, structural studies, and drug discovery efforts targeting CK1 family members. Its role in phosphorylating key components of the Wnt pathway, particularly Dishevelled (DVL) proteins, has made it a focus in cancer research, as dysregulated Wnt signaling is implicated in various malignancies.
CSNK1G2's involvement in neurodegenerative diseases has also gained attention, with studies suggesting connections to tau phosphorylation in Alzheimer's pathology. The recombinant protein enables investigation of kinase-substrate relationships and screening for selective inhibitors. Structural analyses reveal autophosphorylation sites and regulatory mechanisms that modulate its activity. Mutational studies using recombinant variants help map functional domains critical for substrate recognition and membrane association through palmitoylation.
Commercial production of recombinant CSNK1G2 typically involves affinity tag purification (e.g., GST or His-tag) followed by activity validation using specific peptide substrates. Its biochemical properties, including ATP-binding affinity and sensitivity to kinase inhibitors, are routinely characterized to establish assay conditions for high-throughput screening platforms.
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