| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BECN1 |
| Uniprot No | Q14457 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450aa |
| 氨基酸序列 | MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTA QAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLI GEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDT QLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELED VEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSV ENQMRYAQTQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEW NEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELP LYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEK GKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BECN1重组蛋白的3篇代表性文献，按作者和内容概括整理：
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1. **文献名称**：**"Induction of autophagy and inhibition of tumorigenesis by beclin 1"**
**作者**：Liang, X.H., et al. (1999)
**摘要**：该研究首次报道了BECN1(Beclin 1)重组蛋白在自噬调控中的关键作用，通过基因敲除和过表达实验证明其能够诱导自噬并抑制肿瘤形成。研究还利用重组BECN1蛋白验证了其与PI3K复合物的相互作用，为自噬与癌症关联提供了机制基础。
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2. **文献名称**：**"The Beclin 1 network regulates autophagy and apoptosis"**
**作者**：Furuya, N., et al. (2005)
**摘要**：文章解析了重组BECN1蛋白的结构域功能，发现其BH3结构域与抗凋亡蛋白Bcl-2的相互作用可抑制自噬。通过体外重组蛋白结合实验，揭示了BECN1在自噬与凋亡交叉调控中的分子机制。
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3. **文献名称**：**"Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy"**
**作者**：Pattingre, S., et al. (2005)
**摘要**：该研究利用重组BECN1蛋白和Bcl-2家族蛋白进行体外结合实验，证明Bcl-2通过直接结合BECN1的BH3结构域抑制自噬起始，揭示了肿瘤中自噬缺陷的潜在分子机制。
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这些文献涵盖了BECN1重组蛋白在自噬调控、结构功能及疾病机制中的核心研究，适用于自噬相关领域的基础参考。如需更多近期研究，可补充关键词如“BECN1 recombinant protein purification”或“structural analysis”进一步筛选。
**Background of BECN1 Recombinant Protein**
BECN1 (Beclin-1), a key autophagy-related protein encoded by the *BECN1* gene in humans, plays a central role in regulating autophagosome formation and cellular homeostasis. Discovered in 1998 as a Bcl-2-interacting protein, Beclin-1 is a critical component of the class III phosphatidylinositol 3-kinase (PI3K) complex, which drives the nucleation of autophagic vesicles. Its function extends beyond autophagy, influencing processes like apoptosis, endocytosis, and immune responses. Dysregulation of BECN1 is implicated in various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), and infectious diseases, as pathogens often manipulate autophagy pathways for survival.
Recombinant BECN1 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms of the protein for research. This recombinant protein retains functional domains essential for interactions with binding partners, such as the coiled-coil domain for UVRAG/Atg14L binding and the BH3 domain for Bcl-2 association. Researchers utilize it to study autophagy mechanisms, screen for modulators of autophagic flux, and investigate disease pathogenesis. Its applications span structural studies (e.g., crystallography), in vitro binding assays, and cellular rescue experiments in BECN1-deficient models.
The development of BECN1 recombinant protein has advanced therapeutic exploration, particularly in cancer (where autophagy acts as a double-edged sword) and neurodegeneration. It also serves as a tool to dissect pathogen-host interactions, such as viral evasion strategies targeting autophagy. Ongoing research aims to refine its production for enhanced stability and activity, supporting both basic science and drug discovery efforts.
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