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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTGF |
| Uniprot No | P29279 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 253-349aa |
| 氨基酸序列 | MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTGF重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*Recombinant connective tissue growth factor modulates renal epithelial cell behavior*
**作者**：Grotendorst GR 等
**摘要**：研究重组CTGF蛋白对肾上皮细胞迁移和细胞外基质合成的影响，揭示了其在肾脏纤维化中的潜在作用机制。
2. **文献名称**：*Expression and purification of recombinant human CTGF for functional studies*
**作者**：Chen Y 等
**摘要**：报道了一种高效表达和纯化重组人CTGF蛋白的方法，并验证其在体外促进细胞增殖的生物活性，为后续功能研究提供可靠工具。
3. **文献名称**：*CTGF recombinant protein enhances osteoblast differentiation in vitro*
**作者**：Luo Q 等
**摘要**：探讨重组CTGF蛋白通过激活BMP/Smad信号通路促进成骨细胞分化的作用，提示其在骨组织工程中的应用潜力。
(注：以上文献信息为示例性质，实际引用时请核实具体文献内容及来源。)
Connective Tissue Growth Factor (CTGF), also known as CCN2. is a secreted matricellular protein belonging to the CCN family (Cyr61. CTGF, Nov). It plays a pivotal role in regulating cell proliferation, adhesion, migration, and extracellular matrix remodeling. CTGF is strongly implicated in fibrotic disorders, tissue repair, angiogenesis, and tumor progression. Its expression is primarily induced by transforming growth factor-beta (TGF-β), mechanical stress, and hyperglycemia, making it a key mediator in pathological conditions like organ fibrosis, diabetic nephropathy, and cancer metastasis.
Recombinant CTGF proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, biologically active forms for research and therapeutic exploration. These proteins retain functional domains critical for interactions with integrins, heparan sulfate proteoglycans, and growth factor receptors. Researchers utilize recombinant CTGF to study its signaling pathways, including SMAD, MAPK, and PI3K/Akt cascades, and to model fibrosis or tumor microenvironments in vitro and in vivo.
In drug development, recombinant CTGF serves as a tool to screen inhibitors targeting fibrotic pathways or to evaluate its potential as a biomarker. Challenges remain in balancing its dual roles – while CTGF overexpression drives fibrosis and cancer progression, its physiological functions in wound healing and skeletal development are essential. Current studies focus on tissue-specific targeting strategies and understanding context-dependent signaling mechanisms. As an emerging therapeutic target, CTGF recombinant proteins continue to provide insights into complex cell-matrix interactions and precision medicine approaches for fibrosis-related diseases.
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