关键信息
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基因名
ALDH5A1
- 应用
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别名
ALDH5A1；SSADH；Succinate-semialdehyde dehydrogenase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51649
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表达区间
48-535aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMAGRLAGLSAALLRTDSFVGGRWLPAAATF PVQDPASGAALGMVADCGVREARAAVRAAYEAFCRWREVSAKERSSLLRK WYNLMIQNKDDLARIITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYG DIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAMITRKVGAALAAGCTVV VKPAEDTPFSALALAELASQAGIPSGVYNVIPCSRKNAKEVGEAICTDPL VSKISFTGSTTTGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVA GAMASKFRNTGQTCVCSNQFLVQRGIHDAFVKAFAEAMKKNLRVGNGFEE GTTQGPLINEKAVEKVEKQVNDAVSKGATVVTGGKRHQLGKNFFEPTLLC NVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPA QIWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLE LKYVCYGGL
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分子量
55 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ALDH5A1 (aldehyde dehydrogenase 5 family member A1) is an enzyme critically involved in the catabolism of gamma-aminobutyric acid (GABA), playing a vital role in maintaining the balance of neurotransmitters in the brain. Deficiencies in ALDH5A1 are associated with hereditary neurological disorders, such as succinic semialdehyde dehydrogenase deficiency, which can result in developmental delays, seizures, and other significant neurological symptoms. Due to its importance in both metabolic pathways and neurological function, the recombinant production of ALDH5A1 protein has become a focus of research. Utilizing recombinant DNA technology allows for the generation of this enzyme in sufficient quantities for biochemical and structural studies, facilitating a deeper understanding of its function and role in disease. Furthermore, insights gained from this research could lead to the identification of potential therapeutic targets or the development of enzyme replacement therapies for individuals affected by ALDH5A1-related disorders. The expression and purification of recombinant ALDH5A1 provide valuable tools for studying its enzymatic properties, structural features, and interactions with other molecules, thereby contributing to advancements in the treatment and understanding of neurological conditions linked to GABA metabolism. As research continues to unravel the complexities of ALDH5A1, its potential implications in both basic science and clinical applications become increasingly apparent, emphasizing the importance of this enzyme in human health.
