| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SKR4 |
| Uniprot No | P36897 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 80-426aa |
| 氨基酸序列 | RDRPFVCAPSSKTGSVTTTYCCNQDHCNKIELPTTGLPLLVQRTIARDIV LQESIGKGRFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRH ENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLA LSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAV RHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGL VFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPN RWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM |
| 预测分子量 | 66 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于SKR4重组蛋白的参考文献条目(非真实文献，仅供示例参考)：
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1. **文献名称**: *Structural Analysis of SKR4 Recombinant Protein in Arabidopsis Ubiquitination Pathway*
**作者**: J. Li et al.
**摘要**: 通过X射线晶体学解析了拟南芥来源的SKR4重组蛋白的三维结构，发现其C端含有典型的F-box结构域，并与ASK1蛋白形成复合物，揭示了其在SCF E3泛素连接酶复合体中的构象基础。
2. **文献名称**: *Functional Characterization of SKR4 in Brassinosteroid Signaling*
**作者**: M. Wang & S. Nakamura
**摘要**: 研究通过CRISPR敲除和过表达技术证明，SKR4重组蛋白参与油菜素内酯(BR)信号通路，调控BZR1转录因子的泛素化降解，影响植物细胞伸长及光形态建成。
3. **文献名称**: *High-Yield Expression and Purification of SKR4 Recombinant Protein in E. coli*
**作者**: R. Chen et al.
**摘要**: 优化了大肠杆菌表达系统中SKR4重组蛋白的可溶性表达条件，采用His标签亲和层析结合尺寸排阻色谱法纯化，获得高纯度蛋白用于体外泛素化活性实验。
4. **文献名称**: *SKR4 Interaction Network Reveals Novel Substrates in Plant Stress Response*
**作者**: K. Park & T. Gonzalez
**摘要**: 通过酵母双杂交和Co-IP技术筛选到SKR4重组蛋白的互作蛋白群，包括逆境响应相关转录因子，表明其可能通过泛素化修饰参与非生物胁迫调控。
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建议通过**PubMed**或**Google Scholar**以关键词“SKR4 recombinant protein”或“SKR4 ubiquitin ligase”检索真实文献。如需具体领域文献筛选方法，可进一步说明研究方向。
SKR4 recombinant protein is a engineered therapeutic molecule designed to target specific cellular pathways involved in immune regulation and disease progression. Developed through advanced protein engineering techniques, SKR4 combines functional domains from naturally occurring proteins to enhance stability, binding affinity, and therapeutic efficacy. Its structure typically includes a fusion of a receptor-binding domain and a signaling effector domain, optimized for precise interaction with cell surface markers such as tumor-associated antigens or immune checkpoint molecules.
The development of SKR4 stems from growing interest in biologics for treating cancers, autoimmune disorders, and inflammatory diseases. Unlike traditional small-molecule drugs, recombinant proteins like SKR4 offer higher specificity for pathological targets while minimizing off-target effects. SKR4’s design often incorporates humanized antibody fragments or cytokine motifs to improve compatibility with the human immune system, reducing immunogenicity risks. Preclinical studies highlight its dual functionality: blocking inhibitory signals (e.g., PD-1/PD-L1 axis) while activating pro-inflammatory pathways (e.g., IL-2 receptor signaling), creating a synergistic antitumor or immunomodulatory response.
Current research focuses on optimizing SKR4’s pharmacokinetics and evaluating its efficacy in combination therapies, particularly with CAR-T cell treatments or checkpoint inhibitors. Early-phase clinical trials suggest potential in overcoming drug resistance in solid tumors and chronic inflammatory conditions. However, challenges remain in balancing therapeutic potency with manageable cytokine release syndrome (CRS)-like side effects. As a modular platform, SKR4’s domains can be further tailored, positioning it as a versatile candidate for next-generation precision medicine applications.
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