| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPHB3 |
| Uniprot No | P54753 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 585-998aa |
| 氨基酸序列 | QRHGSDSEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKQPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPHB3重组蛋白的3篇参考文献(信息基于公开研究整理，部分为虚构示例，仅供格式参考)：
1. **文献名称**：*"Recombinant EPHB3 extracellular domain inhibits cancer cell migration by binding to ephrin-B ligands"*
**作者**：Chen L, et al.
**摘要**：研究通过大肠杆菌表达系统制备了EPHB3重组胞外域蛋白，验证其与ephrin-B配体的结合能力，并证明其可通过竞争性抑制阻断肿瘤细胞迁移信号通路。
2. **文献名称**：*"Structural and functional analysis of the EPHB3 tyrosine kinase domain produced in mammalian cells"*
**作者**：Smith JR, et al.
**摘要**：利用HEK293细胞表达重组EPHB3激酶域，通过X射线晶体学解析其三维结构，并发现其自磷酸化位点对下游信号传导的关键作用。
3. **文献名称**：*"EPHB3 recombinant protein suppresses angiogenesis in a zebrafish model"*
**作者**：Wang Y, et al.
**摘要**：纯化EPHB3-Fc融合蛋白，通过斑马鱼模型证实其通过抑制VEGF信号通路减少血管生成，为抗肿瘤治疗提供潜在靶点。
(注：以上文献为示例，实际文献需通过PubMed或Web of Science等数据库以关键词“EPHB3 recombinant protein”检索核实。)
**Background of EPHB3 Recombinant Protein**
EPHB3. a member of the Eph receptor tyrosine kinase family, plays critical roles in cell-cell communication, particularly during developmental processes and tissue homeostasis. Eph receptors interact with membrane-bound ephrin ligands to regulate bidirectional signaling pathways that influence cell adhesion, migration, proliferation, and differentiation. EPHB3 is composed of an extracellular ligand-binding domain, a transmembrane region, and a cytoplasmic tyrosine kinase domain. Its activation typically occurs upon binding to ephrin-B ligands, triggering receptor dimerization, autophosphorylation, and downstream signaling cascades. Dysregulation of EPHB3 has been implicated in various pathologies, including cancer, where it may act as either a tumor suppressor or promoter depending on the cellular context. For instance, reduced EPHB3 expression is linked to colorectal cancer progression, while its overexpression in other cancers correlates with metastasis.
Recombinant EPHB3 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the receptor for research and therapeutic applications. This protein retains key structural and functional features, enabling studies on ligand-receptor interactions, signaling mechanisms, and drug discovery. Researchers utilize EPHB3 recombinant protein to develop inhibitors or agonists targeting its activity, investigate its role in disease models, and validate diagnostic or therapeutic antibodies. Its applications extend to understanding neural development, angiogenesis, and epithelial-mesenchymal transition in cancer. By elucidating EPHB3's molecular functions, recombinant forms of the protein contribute to advancing precision medicine strategies for cancers and developmental disorders.
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