Recombinant Human SMPDL3A protein

中文名： 酸性鞘磷脂酶样磷酸二酯酶3a(SMPDL3A)重组蛋白
别名： SMPDL3A；ASML3A；Cyclic GMP-AMP phosphodiesterase SMPDL3A

货号: PA1000-4783

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	SMPDL3A
Uniprot No	Q92484
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	23-453aa
氨基酸序列	LPVAPAGGRNPPPAIGQFWHVTDLHLDPTYHITDDHTKVCASSKGANASN PGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELST DTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVA NLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLN KTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYN EKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPV KSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEY ILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDK TCKAFQICAIMNLDNISYADCLKQLYIKHNYVDHHHHHH
预测分子量	50 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SMPDL3A重组蛋白的3篇参考文献示例(注：部分内容为模拟，建议通过学术数据库核实具体文献)：

---

1. **文献名称**: *SMPDL3A modulates ceramide homeostasis in diabetic kidney disease*

**作者**: Ranganathan, P. et al.

**摘要**: 该研究通过重组SMPDL3A蛋白实验，揭示了其在糖尿病肾病足细胞中调节鞘磷脂代谢的作用。研究发现，重组SMPDL3A可降低细胞毒性鞘氨醇水平，保护足细胞免受高糖诱导的凋亡，提示其潜在治疗价值。

2. **文献名称**: *Structural and functional characterization of SMPDL3A as a sphingomyelinase-like enzyme*

**作者**: Smith, J.L. et al.

**摘要**: 本研究通过重组表达并纯化人源SMPDL3A蛋白，结合X射线晶体学解析其三维结构。功能实验表明，SMPDL3A具有弱酸性鞘磷脂酶活性，且依赖锌离子催化，为开发靶向抑制剂提供了结构基础。

3. **文献名称**: *SMPDL3A as a novel regulator of innate immunity in macrophages*

**作者**: Zhang, Y. et al.

**摘要**: 利用重组SMPDL3A处理巨噬细胞，发现其通过水解细胞膜鞘磷脂，抑制TLR4信号通路并减少促炎因子(如TNF-α、IL-6)释放，表明SMPDL3A在炎症调控中的关键作用。

---

**注意事项**：

- 上述文献为示例性质，实际研究可能需通过PubMed、Google Scholar等平台以关键词“SMPDL3A recombinant protein”检索。

- 推荐关注近年研究，如涉及SMPDL3A与疾病(肾病、癌症)或药物靶点的关联。

背景信息

**Background of SMPDL3A Recombinant Protein**

Sphingomyelin Phosphodiesterase Acid-Like 3A (SMPDL3A) is a membrane-associated glycoprotein belonging to the acid sphingomyelinase-like phosphodiesterase family. It shares structural homology with sphingomyelinases but lacks intrinsic enzymatic activity toward sphingomyelin. Instead, SMPDL3A is implicated in modulating lipid raft organization, cell signaling, and immune responses. The protein is expressed in various tissues, with notable roles in the kidney, immune cells, and endothelial cells.

In renal physiology, SMPDL3A gained attention for its association with podocytes, specialized cells critical for glomerular filtration. Studies suggest it may influence podocyte function and survival, with altered SMPDL3A levels linked to kidney diseases like focal segmental glomerulosclerosis (FSGS). In immune regulation, SMPDL3A interacts with B cells and macrophages, potentially affecting inflammatory pathways and autoimmune conditions.

Recombinant SMPDL3A protein is produced using expression systems (e.g., mammalian, insect, or bacterial cells) to enable functional studies. Its recombinant form retains structural domains, including the conserved metallophosphatase motif, though post-translational modifications (e.g., glycosylation) vary by expression host. Researchers utilize this protein to investigate its role in lipid metabolism, membrane repair, and immune modulation, as well as to explore therapeutic strategies targeting SMPDL3A in kidney or inflammatory disorders.

Current research also examines SMPDL3A’s potential as a biomarker or therapeutic target, particularly in contexts where lipid signaling or membrane integrity is disrupted. Its dual roles in cellular homeostasis and disease pathology make it a protein of growing interest in nephrology and immunology.