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Rabbit Polyclonal MouseIrf3(N-term) Antibody

  • 中文名： Mouse Irf3 (N-term)抗体
  • 别    名： Interferon regulatory factor 3, IRF-3, Irf3
货号: IPDX32349
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesInterferon regulatory factor 3, IRF-3, Irf3
Entrez GeneID54131
WB Predicted band size46.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityMouse
ImmunogenThis Mouse Irf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the N-terminal region of mouse Irf3.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

1. **"IRF3 mediates a TLR3/TLR4-specific antiviral gene program"**

*作者: Sato M. 等*

摘要：研究利用针对IRF3 N端的抗体，通过免疫沉淀和Western blot分析，揭示了IRF3在TLR3/4信号通路中激活抗病毒基因的关键作用。

2. **"Distinct roles of IRF3 and IRF7 in IFN-β gene regulation"**

*作者: Honda K. 等*

摘要：通过N端特异性抗体进行染色质免疫共沉淀(ChIP)，证实IRF3在病毒刺激下优先结合IFN-β启动子，与IRF7协同调控先天免疫应答。

3. **"Phosphorylation-induced IRF3核转位机制研究"**

*作者: Chen W. 等*

摘要：使用Mouse Irf3 (N-term)抗体进行免疫荧光实验，揭示了IRF3磷酸化后从胞质转位至细胞核的分子机制，及其在抗病毒反应中的动态变化。

4. **"Irf3-deficient mice reveal essential type I interferon responses"**

*作者: Taniguchi T. 等*

摘要：通过Western blot和免疫组化(使用N端抗体)验证Irf3基因敲除模型，证明IRF3缺失导致I型干扰素产生缺陷，影响宿主对RNA病毒的抗性。

(注：以上为示例文献，实际引用时需核对真实论文信息。)

背景信息

The Mouse Irf3 (N-term) antibody is a specific immunological tool designed to detect the N-terminal region of interferon regulatory factor 3 (IRF3) in mouse-derived samples. IRF3. a transcription factor in the IRF family, plays a pivotal role in innate immune responses, particularly in antiviral signaling pathways. Upon viral infection or pattern recognition receptor (PRR) activation (e.g., TLR3. RIG-I), IRF3 undergoes phosphorylation and conformational changes, leading to its dimerization and nuclear translocation. In the nucleus, IRF3 binds to interferon-stimulated response elements (ISREs) to drive the expression of type I interferons (IFN-α/β) and proinflammatory cytokines. The N-terminal domain of IRF3 contains critical functional motifs, including the DNA-binding domain and regulatory regions essential for its activation and interaction with cofactors like TBK1 or IKKε. Antibodies targeting this region are widely used in applications such as Western blotting, immunofluorescence, and immunoprecipitation to study IRF3 expression, post-translational modifications (e.g., phosphorylation at Ser386/Ser396), and subcellular localization during immune activation. Their specificity makes them valuable for investigating IRF3's role in viral infections, autoimmune diseases, and cancer immunology, as well as validating IRF3 knockout or knockdown models. Proper controls (e.g., IRF3-deficient cells) are recommended to confirm antibody specificity due to potential cross-reactivity with other IRF family members.

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