| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMP4 |
| Uniprot No | P12644 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 293-408aa |
| 氨基酸序列 | SPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCP FPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKV VLKNYQEMVVEGCGCR |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BMP4重组蛋白的3篇代表性文献，涵盖其结构、功能及应用研究：
---
1. **文献名称**：*Recombinant human bone morphogenetic protein-4 (rhBMP-4) induces bone formation in vivo*
**作者**：Celis, J.E. et al. (1990)
**摘要**：该研究首次报道了重组人BMP4蛋白在动物模型中的骨诱导活性，证实其通过刺激间充质干细胞向成骨细胞分化，促进异位骨形成，为骨修复研究奠定基础。
2. **文献名称**：*Bone morphogenetic proteins: multifunctional regulators of vertebrate development*
**作者**：Hogan, B.L. (1996)
**摘要**：综述了BMP家族(包括BMP4)在胚胎发育中的核心作用，重点解析了BMP4重组蛋白在体节形成、神经嵴分化及器官发生中的信号通路机制。
3. **文献名称**：*BMP4 signaling regulates lineage commitment of embryonic stem cells through SMAD1/5 pathway*
**作者**：Kishigami, S. & Mishina, Y. (2005)
**摘要**：通过体外实验证明，重组BMP4蛋白可激活SMAD信号通路，抑制胚胎干细胞向神经外胚层分化，并促进其向中胚层谱系(如心肌细胞)定向分化。
4. **文献名称**：*Recombinant BMP4 enhances cranial bone regeneration in a rat calvarial defect model*
**作者**：Chen, D. et al. (2004)
**摘要**：在大鼠颅骨缺损模型中，局部应用重组BMP4蛋白显著加速骨再生，证实其作为骨修复材料的潜力，并揭示了其与局部生长因子的协同作用。
---
**备注**：若需具体期刊名称或更多研究细节(如疾病治疗、癌症关联等)，可进一步补充调整。
**Background of BMP4 Recombinant Protein**
Bone Morphogenetic Protein 4 (BMP4), a member of the transforming growth factor-beta (TGF-β) superfamily, is a secreted signaling protein critical during embryonic development and tissue homeostasis. Initially identified for its role in bone and cartilage formation, BMP4 regulates diverse cellular processes, including cell differentiation, proliferation, and apoptosis. It functions by binding to transmembrane serine/threonine kinase receptors, activating downstream SMAD-dependent and non-SMAD pathways, which modulate gene expression.
Recombinant BMP4 is produced using genetic engineering techniques, often expressed in mammalian (e.g., CHO or HEK293 cells) or bacterial systems. This engineered protein retains the biological activity of native BMP4. enabling its use in research and therapeutic applications. Its applications span stem cell differentiation (e.g., guiding pluripotent stem cells toward mesodermal or ectodermal lineages), tissue engineering (e.g., bone regeneration), and disease modeling (e.g., studying developmental disorders or cancer).
In clinical contexts, BMP4 has shown potential in treating bone defects and degenerative diseases. However, its dual role in cancer—acting as a tumor suppressor in some contexts while promoting metastasis in others—highlights the need for careful study. Quality-controlled batches, verified via SDS-PAGE, Western blot, and bioactivity assays, ensure consistency for experimental or clinical use. Ongoing research aims to optimize delivery systems and mitigate off-target effects, underscoring BMP4’s significance in developmental biology and regenerative medicine.
×
