| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTR8 |
| Uniprot No | Q9H981-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 氨基酸序列 | MNHKVMILMKMGFSGIVVHQESVCATYGSGLSSTCIVDVGDQKTSVCCVE DGVSHRNTRIFSWNQDISGLQDHEFQIRHPDSPALLYQFRLGDEKLQAPM ALFYPATFGIVGQKMTTLQHRSQGDPEDPHDEHYLLATQSKQEQSAKATA DRKSASKPIGFEGDLRGQSSDLPERLHSQEVDLGSAQGDGLMAGNDSEEA LTALMSRKTAISLFEGKALGLDKAILHSIDCCSSDDTKKKMYSSILVVGG GLMFHKAQEFLQHRILNKMPPSFRRIIENVDVITRPKDMDPRLIAWKGGA VLACLDTTQELWIYQREWQ RFGVRMLRERAAFVW |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于ACTR8重组蛋白的研究目前公开文献较少，以下为基于相关领域推测的模拟示例(实际文献可能需要进一步验证)：
1. **标题**：Structural and Functional Analysis of ACTR8 in Chromatin Remodeling Complexes
**作者**：Chen L, et al.
**摘要**：本研究通过重组表达人源ACTR8蛋白，解析了其与染色质重塑复合体其他亚基的相互作用，发现ACTR8在调控组蛋白乙酰化和DNA可及性中起关键作用。
2. **标题**：Recombinant ACTR8 Expression and Purification for Biochemical Characterization
**作者**：Wang Y, et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化ACTR8重组蛋白的方法，并通过体外实验验证其参与调控核小体结构的稳定性。
3. **标题**：ACTR8 Gene Knockout Reveals Its Role in DNA Damage Repair
**作者**：Kimura T, et al.
**摘要**：利用CRISPR技术构建ACTR8敲除细胞系，发现ACTR8缺失导致DNA损伤修复效率下降，提示其与同源重组修复通路相关。
**注意事项**：
- 上述内容为模拟示例，实际研究可能有限，建议通过PubMed或Google Scholar以“ACTR8 recombinant protein”“ACTR8 function”为关键词检索最新文献。
- 若研究涉及其他名称(如ARP复合体相关蛋白)，需确认基因/蛋白命名是否一致。
**Background of ACTR8 Recombinant Protein**
ACTR8 (Actin-Related Protein 8) is a conserved subunit of chromatin-modifying complexes, notably the NuA4/TIP60 complex, which plays a critical role in transcriptional regulation, DNA repair, and chromatin remodeling. This complex acetylates histones, particularly H4 and H2A, to facilitate transcriptional activation and maintain genomic stability. ACTR8 contributes to structural integrity and functional specificity of these complexes, enabling interactions with other regulatory proteins or DNA substrates.
As a recombinant protein, ACTR8 is engineered for in vitro studies to dissect its molecular functions. Recombinant expression systems (e.g., *E. coli*, mammalian cells) allow high-yield production of purified ACTR8. often tagged with affinity markers (e.g., His-tag) for ease of isolation. This approach enables detailed biochemical assays, such as analyzing acetyltransferase activity, protein-protein interactions, or structural dynamics within the NuA4/TIP60 complex.
Research highlights ACTR8’s involvement in cellular processes like cell cycle regulation, apoptosis, and oncogenesis. Dysregulation of NuA4/TIP60 is linked to cancers, neurodegenerative diseases, and developmental disorders, positioning ACTR8 as a potential therapeutic target. Recombinant ACTR8 also aids in drug discovery, serving as a tool to screen inhibitors of chromatin-modifying enzymes.
In summary, recombinant ACTR8 provides a versatile platform to explore epigenetic mechanisms, advancing both basic science and translational applications in disease biology.
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