| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRADC1 |
| Uniprot No | Q9BSG0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-188aa |
| 氨基酸序列 | HGFRIHDYLYFQVLSPGDIRYIFTATPAKDFGGIFHTRYEQIHLVPAEPP EACGELSNGFFIQDQIALVERGGCSFLSKTRVVQEHGGRAVIISDNAVDN DSFYVEMIQDSTQRTADIPALFLLGRDGYMIRRSLEQHGLPWAIISIPVN VTSIPTFELLQPPWTFWVDHHHHHH |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRADC1重组蛋白的假设性参考文献示例，概括了不同研究方向的内容：
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1. **标题**：*Expression and Purification of Recombinant PRADC1 in Escherichia coli*
**作者**：Zhang Y, Wang L, et al.
**摘要**：本研究成功构建了PRADC1的重组表达载体，并利用大肠杆菌系统高效表达可溶性PRADC1蛋白。通过亲和层析技术纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **标题**：*Structural Insights into PRADC1 Revealed by X-ray Crystallography*
**作者**：Smith J, Brown K, et al.
**摘要**：通过重组PRADC1蛋白的结晶和X射线衍射分析，首次解析了其三维结构，揭示了其C端结构域中关键的脯氨酸富集区域，暗示其可能参与蛋白相互作用。
3. **标题**：*PRADC1 Recombinant Protein Modulates Apoptosis in Cancer Cells*
**作者**：Lee S, Kim M, et al.
**摘要**：体外实验表明，重组PRADC1蛋白能通过调控Bcl-2家族蛋白的表达，抑制肿瘤细胞凋亡，提示其在癌症治疗中的潜在作用靶点。
4. **标题**：*Functional Characterization of PRADC1 in Inflammatory Signaling*
**作者**：Chen X, Liu R, et al.
**摘要**：利用重组PRADC1蛋白进行细胞实验，发现其通过NF-κB通路调节炎症因子释放，为自身免疫疾病的机制研究提供了新方向。
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注：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。
**Background of PRADC1 Recombinant Protein**
PRADC1 (Partner of RAD21 Coiled-Coil Protein 1) is a protein encoded by the *PRADC1* gene, primarily recognized for its role in cellular processes linked to chromosome segregation and genomic stability. It interacts with RAD21. a core component of the cohesin complex, which is essential for sister chromatid cohesion during mitosis and meiosis. This interaction suggests PRADC1’s involvement in maintaining chromosomal integrity, DNA repair, and proper cell cycle progression. Dysregulation of cohesin-related proteins, including PRADC1. has been implicated in developmental disorders and cancers, highlighting its biological significance.
Recombinant PRADC1 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the protein. Its recombinant version retains key structural domains, such as coiled-coil motifs, which mediate protein-protein interactions critical for cohesin complex assembly and function. Researchers utilize this tool to study PRADC1’s molecular mechanisms, including its binding partners, post-translational modifications, and role in chromatin organization.
In biomedical research, PRADC1 recombinant protein serves as a reagent for *in vitro* assays, antibody production, and structural studies. It aids in exploring cohesinopathies (e.g., Cornelia de Lange syndrome) and cancer biology, where cohesin dysfunction contributes to aneuploidy or transcriptional misregulation. Additionally, it supports drug discovery efforts targeting cohesin-related pathways.
Despite its emerging importance, PRADC1’s full functional spectrum remains under investigation. The availability of recombinant PRADC1 accelerates mechanistic studies, offering insights into its contribution to cellular homeostasis and disease pathogenesis.
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