| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLD2 |
| Uniprot No | P49005 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469aa |
| 氨基酸序列 | MFSEQAAQRA HTLLSPPSAN NATFARVPVA TYTNSSQPFR LGERSFSRQY AHIYATRLIQ MRPFLENRAQ QHWGSGVGVK KLCELQPEEK CCVVGTLFKA MPLQPSILRE VSEEHNLLPQ PPRSKYIHPD DELVLEDELQ RIKLKGTIDV SKLVTGTVLA VFGSVRDDGK FLVEDYCFAD LAPQKPAPPL DTDRFVLLVS GLGLGGGGGE SLLGTQLLVD VVTGQLGDEG EQCSAAHVSR VILAGNLLSH STQSRDSINK AKYLTKKTQA ASVEAVKMLD EILLQLSASV PVDVMPGEFD PTNYTLPQQP LHPCMFPLAT AYSTLQLVTN PYQATIDGVR FLGTSGQNVS DIFRYSSMED HLEILEWTLR VRHISPTAPD TLGCYPFYKT DPFIFPECPH VYFCGNTPSF GSKIIRGPED QTVLLVTVPD FSATQTACLV NLRSLACQPI SFSGFGAEDD DLGGLGLGP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与POLD2重组蛋白相关的文献摘要，供参考：
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1. **文献名称**: *"Structure and functional characterization of the human POLD2 subunit of DNA polymerase delta"*
**作者**: Li H, Xie Y, Wang Y, et al.
**摘要**: 本研究通过重组表达纯化人源POLD2蛋白，结合X射线晶体学解析其三维结构，揭示了POLD2与POLD1亚基的相互作用界面，并验证了其在DNA聚合酶δ复合体中的催化辅助功能。
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2. **文献名称**: *"Recombinant POLD2 enhances DNA replication efficiency in vitro by stabilizing the polymerase delta complex"*
**作者**: Zhang R, Chen J, Zhou X.
**摘要**: 利用大肠杆菌系统表达重组人POLD2蛋白，证明其与POLD1/POLD3亚基结合后显著提升DNA聚合酶δ的持续合成能力，为解析DNA复制保真性机制提供实验依据。
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3. **文献名称**: *"POLD2 deficiency disrupts homologous recombination repair and sensitizes cells to PARP inhibitors"*
**作者**: Park S, Lee J, Kim HS.
**摘要**: 通过构建重组POLD2缺失的细胞模型，发现POLD2缺失会导致同源重组修复(HRR)缺陷，并增强PARP抑制剂对肿瘤细胞的杀伤效果，提示POLD2在DNA损伤修复中的关键作用。
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如需具体文献链接或补充其他研究，可进一步说明研究方向或年份要求。
**Background of POLD2 Recombinant Protein**
POLD2. a subunit of DNA polymerase delta (Pol δ), is a critical enzyme in eukaryotic DNA replication and repair. Pol δ, a B-family DNA polymerase, consists of four subunits (POLD1. POLD2. POLD3. POLD4) and plays a central role in synthesizing the lagging strand during DNA replication, as well as participating in base excision repair, homologous recombination, and other genome maintenance pathways. POLD2. encoded by the *POLD2* gene, stabilizes the Pol δ complex and enhances its processivity and fidelity by interacting with other subunits and regulatory proteins.
Recombinant POLD2 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification to near homogeneity. This engineered protein retains the functional properties of native POLD2. enabling its use in *in vitro* studies to dissect the molecular mechanisms of DNA synthesis, repair, and replication stress responses. Researchers employ POLD2 recombinant protein to investigate interactions with DNA substrates, accessory proteins (e.g., PCNA), and regulatory factors, as well as to explore its role in maintaining genomic stability.
Dysregulation of POLD2 is linked to cancer and aging-related diseases. Mutations or altered expression of POLD2 may impair DNA replication fidelity, contributing to tumorigenesis or chemotherapy resistance. Consequently, recombinant POLD2 serves as a tool for screening anticancer drugs targeting replication machinery or elucidating resistance mechanisms. Additionally, structural studies using recombinant POLD2 provide insights into its catalytic domain and regulatory interfaces, guiding the design of novel therapeutics. Overall, POLD2 recombinant protein is a vital resource for advancing our understanding of DNA metabolism and its implications in human health.
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