关键信息
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基因名
CRHBP
- 应用
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别名
CRHBP；CRFBP;Corticotropin-releasing factor-binding Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P24387
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表达区间
25-322aa
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氨基酸序列
YLELREAADYDPFLLFSANLKRELAGEQPYRRALRCLDMLSLQGQFTFTA DRPQLHCAAFFISEPEEFITIHYDQVSIDCQGGDFLKVFDGWILKGEKFP SSQDHPLPSAERYIDFCESGLSRRSIRSSQNVAMIFFRVHEPGNGFTLTI KTDPNLFPCNVISQTPNGKFTLVVPHQHRNCSFSIIYPVVIKISDLTLGH VNGLQLKKSSAGCEGIGDFVELLGGTGLDPSKMTPLADLCYPFHGPAQMK VGCDNTVVRMVSSGKHVNRVTFEYRQLEPYELENPNGNSIGEFCLSGLVD HHHHHH
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分子量
34 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CRHBP (Corticotropin-Releasing Hormone Binding Protein) is essential in the regulation of the hypothalamic-pituitary-adrenal (HPA) axis and plays a significant role in the stress response. As a high-affinity binding protein for corticotropin-releasing hormone (CRH), CRHBP modulates the availability of CRH, thereby influencing the secretion of adrenocorticotropic hormone (ACTH) and cortisol. Dysregulation of this pathway has been implicated in various psychological disorders, including depression, anxiety, and post-traumatic stress disorder (PTSD). Consequently, understanding CRHBP's structure and function is crucial for developing therapeutic strategies targeting stress-related conditions. Recent advancements in recombinant protein technology have enabled the production of CRHBP in vitro, providing a valuable tool for studying its characteristics and interactions. These studies focus on the protein's affinity, structure-activity relationships, and its role in binding to CRH. Additionally, investigations into CRHBP's potential as a biomarker for stress-related disorders and as a therapeutic target have gained momentum. Overall, research on recombinant CRHBP promises to enrich our understanding of the neuroendocrine mechanisms underlying stress responses and offers new avenues for intervention in mental health disorders.
