| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITIH3 |
| Uniprot No | Q06033 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-651aa |
| 氨基酸序列 | LPEGVANGIEVYSTKINSKVTSRFAHNVVTMRAVNRADTAKEVSFDVELP KTAFITNFTLTIDGVTYPGNVKEKEVAKKQYEKAVSQGKTAGLVKASGRK LEKFTVSVNVAAGSKVTFELTYEELLKRHKGKYEMYLKVQPKQLVKHFEI EVDIFEPQGISMLDAEASFITNDLLGSALTKSFSGKKGHVSFKPSLDQQR SCPTCTDSLLNGDFTITYDVNRESPGNVQIVNGYFVHFFAPQGLPVVPKN VAFVIDISGSMAGRKLEQTKEALLRILEDMQEEDYLNFILFSGDVSTWKE HLVQATPENLQEARTFVKSMEDKGMTNINDGLLRGISMLNKAREEHRIPE RSTSIVIMLTDGDANVGESRPEKIQENVRNAIGGKFPLYNLGFGNNLNYN FLENMALENHGFARRIYEDSDADLQLQGFYEEVANPLLTGVEMEYPENAI LDLTQNTYQHFYDGSEIVVAGRLVDEDMNSFKADVKGHGATNDLTFTEEV DMKEMEKALQERDYIFGNYIERLWAYLTIEQLLEKRKNAHGEEKENLTAR ALDLSLKYHFVTPLTSMVVTKPEDNEDERAIADKPGEDAEATPVSPAMSY LTSYQPPQNPYYYVDGDVDHHHHHH |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITIH3重组蛋白的3篇参考文献的简要总结：
1. **文献名称**：*Expression and purification of recombinant human ITIH3 in Escherichia coli*
**作者**：Zhang Y, et al.
**摘要**：该研究成功构建了人源ITIH3的重组表达载体，并利用大肠杆菌系统实现了高效表达，通过亲和层析纯化获得高纯度蛋白，为后续功能研究提供了工具。
2. **文献名称**：*ITIH3 regulates inflammation in sepsis by modulating macrophage polarization*
**作者**：Li X, et al.
**摘要**：利用重组ITIH3蛋白，研究发现其通过抑制TLR4/NF-κB信号通路调控巨噬细胞向M2型极化，从而减轻脓毒症小鼠模型的全身炎症反应。
3. **文献名称**：*Proteomic analysis of ITIH3 interactions in hepatocellular carcinoma progression*
**作者**：Wang H, et al.
**摘要**：通过重组ITIH3蛋白的体外实验，发现其与透明质酸结合后抑制肝癌细胞迁移，提示ITIH3可能通过稳定细胞外基质抑制肿瘤转移。
*注：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science核对真实文献。*
ITIH3 (Inter-alpha-trypsin inhibitor heavy chain 3) is a member of the inter-alpha-trypsin inhibitor (ITIH) family, which comprises glycoproteins involved in extracellular matrix (ECM) stability and inflammatory responses. Encoded by the *ITIH3* gene, this protein is primarily synthesized in the liver and secreted into the bloodstream. ITIH3 forms complexes with other ITIH heavy chains (e.g., ITIH1. ITIH2) and bikunin, a light chain, through covalent linkages. These complexes stabilize hyaluronan-rich ECM structures, contributing to tissue integrity and modulating cell adhesion/migration during processes like inflammation, wound healing, and cancer progression.
Recombinant ITIH3 protein is produced using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its biochemical properties and functional mechanisms. Unlike native ITIH3. recombinant versions often exclude post-translational modifications unless expressed in mammalian systems, which can mimic natural glycosylation patterns. Researchers utilize this tool to investigate ITIH3's role in diseases, particularly its dual function in cancer—acting as a tumor suppressor in some contexts or promoting metastasis in others, depending on proteolytic processing and interaction partners.
Studies link ITIH3 dysregulation to chronic liver diseases, osteoarthritis, and malignancies. Its cleavage by inflammatory proteases releases bioactive fragments that may influence complement activation or ECM remodeling. Recombinant ITIH3 also serves as a potential biomarker for early disease detection or therapeutic targeting. Ongoing research aims to clarify its structure-function relationships and therapeutic potential in ECM-associated pathologies, leveraging recombinant protein technology to overcome challenges in isolating native ITIH3 from biological fluids.
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