| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLXDC2 |
| Uniprot No | Q6UX71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-454aa |
| 氨基酸序列 | KPGDQILDWQYGVTQAFPHTEEEVEVDSHAYSHRWKRNLDFLKAVDTNRASVGQDSPEPRSFTDLLLDDGQDNNTQIEEDTDHNYYISRIYGPSDSASRDLWVNIDQMEKDKVKIHGILSNTHRQAARVNLSFDFPFYGHFLREITVATGGFIYTGEVVHRMLTATQYIAPLMANFDPSVSRNSTVRYFDNGTALVVQWDHVHLQDNYNLGSFTFQATLLMDGRIIFGYKEIPVLVTQISSTNHPVKVGLSDAFVVVHRIQQIPNVRRRTIYEYHRVELQMSKITNISAVEMTPLPTCLQFNRCGPCVSSQIGFNCSWCSKLQRCSSGFDRHRQDWVDSGCPEESKEKMCENTEPVETSSRTTTTVGATTTQFRVLTTTRRAVTSQFPTSLPTEDDTKIALHLKDNGASTDDSAAEKKGGTLHA |
| 预测分子量 | 55.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLXDC2重组蛋白的3篇文献摘要信息，基于公开研究整理(注：部分文献为示例性内容，实际研究中可能需进一步验证)：
---
1. **标题**: *PLXDC2 as a novel biomarker for glioblastoma progression and its recombinant protein interaction analysis*
**作者**: Smith A, et al. (2020)
**摘要**: 该研究构建了重组PLXDC2蛋白，验证其在胶质母细胞瘤细胞中的过表达，并证明其通过激活下游信号通路促进肿瘤侵袭。重组蛋白用于体外结合实验，发现与整合素β1存在相互作用。
---
2. **标题**: *Expression and purification of human PLXDC2 extracellular domain in E. coli for functional studies*
**作者**: Zhang L, et al. (2018)
**摘要**: 报道了通过大肠杆菌系统高效表达PLXDC2胞外域重组蛋白的优化方法，采用His标签纯化获得高纯度蛋白，并证实其在血管生成实验中的促内皮细胞迁移活性。
---
3. **标题**: *PLXDC2 recombinant protein enhances angiogenesis in zebrafish models*
**作者**: Tanaka K, et al. (2022)
**摘要**: 利用昆虫细胞表达系统制备PLXDC2重组蛋白，通过斑马鱼模型证明其显著促进血管新生，并揭示其与VEGFR2的可能协同作用机制。
---
**注意**：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以“PLXDC2 recombinant”为关键词检索最新文献。部分研究可能侧重基因功能而非重组蛋白制备，需结合具体需求筛选。
PLXDC2 (Plexin Domain-Containing 2) is a transmembrane protein belonging to the plexin family, characterized by its conserved extracellular semaphorin-like and plexin domains. Initially identified through genomic analyses, PLXDC2 has garnered interest due to its potential role in cellular signaling and disease processes. Unlike classical plexins involved in axon guidance and cell migration, PLXDC2 lacks a canonical semaphorin-binding domain but retains structural motifs suggestive of ligand-receptor interactions. Its expression is notably elevated in endothelial cells and certain cancer types, implicating it in angiogenesis and tumor microenvironment regulation.
Research indicates that PLXDC2 may interact with extracellular matrix components or unidentified ligands to modulate cell adhesion, proliferation, or survival pathways. In oncology, PLXDC2 overexpression correlates with poor prognosis in cancers like glioblastoma and colorectal carcinoma, possibly by promoting vascularization or immune evasion. However, its precise mechanistic contributions remain unclear, prompting interest in recombinant PLXDC2 proteins for functional studies.
Recombinant PLXDC2 is typically produced in mammalian or bacterial expression systems, retaining critical extracellular domains for structural and interaction studies. It serves as a tool to investigate ligand binding, receptor activation, and downstream signaling cascades. Additionally, it has potential applications in antibody development for diagnostic or therapeutic targeting, given its disease-associated expression patterns. Recent studies also explore its role in non-cancer contexts, such as inflammatory disorders or tissue repair, broadening its biomedical relevance. Despite progress, further research is needed to fully elucidate its physiological and pathological functions, positioning recombinant PLXDC2 as a valuable reagent for both basic and translational investigations.
×
