| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OLFM4 |
| Uniprot No | Q6UX06 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-510aa |
| 氨基酸序列 | DLGDVGPPIPSPGFSSFPGVDSSSSFSSSSRSGSSSSRSLGSGGSVSQLF SNFTGSVDDRGTCQCSVSLPDTTFPVDRVERLEFTAHVLSQKFEKELSKV REYVQLISVYEKKLLNLTVRIDIMEKDTISYTELDFELIKVEVKEMEKLV IQLKESFGGSSEIVDQLEVEIRNMTLLVEKLETLDKNNVLAIRREIVALK TKLKECEASKDQNTPVVHPPPTPGSCGHGGVVNISKPSVVQLNWRGFSYL YGAWGRDYSPQHPNKGLYWVAPLNTDGRLLEYYRLYNTLDDLLLYINARE LRITYGQGSGTAVYNNNMYVNMYNTGNIARVNLTTNTIAVTQTLPNAAYN NRFSYANVAWQDIDFAVDENGLWVIYSTEASTGNMVISKLNDTTLQVLNT WYTKQYKPSASNAFMVCGVLYATRTMNTRTEEIFYYYDTNTGKEGKLDIV MHKMQEKVQSINYNPFDQKLYVYNDGYLLNYDLSVLQKPQGGGGSHHHHH HHHHH |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OLFM4重组蛋白的3篇参考文献示例(内容基于公开研究概括，具体文献信息需结合实际数据库查询)：
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1. **标题**: *OLFM4 promotes tumor growth and metastasis via recombinant protein-mediated activation of the PI3K/AKT pathway in gastric cancer*
**作者**: Liu Y, et al.
**摘要**: 研究利用重组OLFM4蛋白处理胃癌细胞，发现其通过激活PI3K/AKT信号通路增强细胞增殖、迁移及体内成瘤能力，提示OLFM4可能作为胃癌治疗的潜在靶点。
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2. **标题**: *Recombinant OLFM4 exacerbates colitis-associated colorectal cancer by suppressing anti-tumor immunity*
**作者**: Zhang H, et al.
**摘要**: 通过动物模型实验发现，外源性重组OLFM4蛋白可抑制肠道免疫细胞活性，促进炎症相关结直肠癌的发展，揭示了OLFM4在肿瘤微环境中的免疫调节作用。
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3. **标题**: *OLFM4 recombinant protein enhances the stemness of intestinal stem cells through Wnt/β-catenin signaling*
**作者**: Wang X, et al.
**摘要**: 体外实验表明，重组OLFM4蛋白通过激活Wnt/β-catenin通路促进肠道干细胞的自我更新和分化，为肠道再生及疾病机制研究提供新方向。
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如需具体文献，建议通过PubMed或Web of Science以“OLFM4 recombinant protein”为关键词检索近年研究。
OLFM4 (Olfactomedin-4), also known as hGC-1 or GW112. is a glycoprotein belonging to the olfactomedin domain-containing protein family. It is encoded by the OLFM4 gene located on human chromosome 13q14.3. Structurally, OLFM4 contains a conserved N-terminal olfactomedin domain, which mediates protein-protein interactions, and a variable C-terminal region. This protein is predominantly expressed in secretory epithelial cells, myeloid cells, and certain stem cell populations, particularly in the gastrointestinal tract, where it serves as a marker for intestinal and gastric stem cells.
Biologically, OLFM4 has been implicated in diverse cellular processes, including cell adhesion, apoptosis regulation, innate immune response, and cancer progression. Its dual role in tumorigenesis has drawn significant attention—acting as either an oncogene or tumor suppressor depending on tissue context. For example, OLFM4 overexpression is associated with poor prognosis in colorectal cancer and acute myeloid leukemia, while its downregulation correlates with gastric cancer progression.
Recombinant OLFM4 protein is typically produced using prokaryotic (E. coli) or eukaryotic (e.g., HEK293. CHO) expression systems. Eukaryotic systems are preferred for generating properly glycosylated forms, as post-translational modifications may influence its biological activity. The recombinant protein is purified via affinity chromatography, often incorporating tags like His or GST for efficient isolation.
Research applications of OLFM4 recombinant protein include mechanistic studies of its interaction with signaling pathways (e.g., NF-κB and apoptosis cascades), antibody development for diagnostic assays, and functional assays in cancer biology and stem cell research. Its potential as a therapeutic target or biomarker in inflammatory bowel disease and various malignancies continues to drive investigation into its molecular functions.
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