Cat: IPD-X41736

Recombinant Escherichia coli recT Protein ,His & Myc

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关键信息

  • 基因名

    recT

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    (P33)

  • 种属

    Escherichia coli

  • 表达系统

    E. coli

  • 标签

    N- His & C- Myc

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P33228

  • 表达区间

    1-269aa

  • 分子量

    37.2 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

RecT is a pivotal protein involved in the homologous recombination repair pathway in bacteria, particularly in organisms like Escherichia coli. As a member of the RecT family, this protein plays a crucial role in facilitating the integration of single-stranded DNA into homologous double-stranded DNA, a process essential for DNA repair, genetic diversity, and horizontal gene transfer. The study of RecT has gained significant interest due to its potential applications in biotechnology and genetic engineering. By harnessing the unique properties of RecT, researchers aim to develop innovative tools for targeted gene editing, enhancing transformation efficiency, and investigating the underlying mechanisms of recombination. Furthermore, understanding the structural and functional dynamics of RecT can provide insights into bacterial evolution and the mechanisms of antibiotic resistance. With advancements in molecular biology techniques, recent studies have focused on characterizing the interaction of RecT with other proteins involved in recombination and DNA repair pathways, as well as its regulation under different cellular conditions. The exploration of RecT not only sheds light on fundamental biological processes but also paves the way for the development of novel therapeutic approaches and genetic manipulation strategies in various organisms.

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