| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MANSC1 |
| Uniprot No | Q9H8J5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MFFGGEGSLTYTLVIICFLTLRLSASQNCLKKSLEDVVIDIQSSLSKGIRGNEPVYTSTQEDCINSCCSTKNISGDKACNLMIFDTRKTARQPNCYLFFCPNEEACPLKPAKGLMSYRIITDFPSLTRNLPSQELPQEDSLLHGQFSQAVTPLAHHHTDYSKPTDISWRDTLSQKFGSSDHLEKLFKMDEASAQLLAYKEKGHSQSSQFSSDQEIAHLLPENVSALPATVAVASPHTTSATPKPATLLPTNASVTPSGTSQPQLATTAPPVTTVTSQPPTTLISTVFTRAAATLQAMATTAVLTTTFQAPTDSKGSLETIPFTEISNLTLNTGNVYNPTALSMSNVESSTMNKTASWEGREASPGSSSQGSVPENQYGLPFEKWLLIGSLLFGVLFLVIGLVLLGRILSESLRRKRYSRLDYLINGIYVDI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MANSC1重组蛋白的3篇代表性文献摘要概览：
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1. **文献名称**: *Characterization of MANSC1 as a novel regulator of extracellular matrix remodeling in cancer*
**作者**: Smith A, et al. (2022)
**摘要**: 本研究首次报道了重组人MANSC1蛋白在肿瘤微环境中的作用。通过大肠杆菌表达系统纯化MANSC1重组蛋白，发现其能够抑制基质金属蛋白酶(MMP2/9)活性，并通过阻断整合素信号通路抑制癌细胞侵袭。揭示了MANSC1在细胞外基质重塑中的潜在调控机制。
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2. **文献名称**: *Recombinant MANSC1 protein attenuates LPS-induced inflammatory response via modulating TLR4/NF-κB signaling*
**作者**: Wang Y, et al. (2020)
**摘要**: 研究团队利用哺乳动物HEK293细胞系统表达带His标签的MANSC1重组蛋白，验证其在内毒素(LPS)诱导的巨噬细胞炎症模型中的功能。结果表明，重组MANSC1通过竞争性结合TLR4受体，抑制NF-κB通路活化，显著降低IL-6和TNF-α的分泌，提示其抗炎治疗潜力。
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3. **文献名称**: *Structural and functional analysis of the Manscan domain in MANSC1 protein*
**作者**: Gonzalez R, et al. (2018)
**摘要**: 该研究通过昆虫细胞表达系统获得高纯度MANSC1重组蛋白，利用X射线晶体学解析其Manscan结构域的三维结构(分辨率2.1Å)。功能实验表明该结构域具有钙离子依赖性黏附分子结合特性，为理解MANSC1在胚胎发育中的生物学功能提供了结构基础。
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注：以上文献信息为示例性质，若需真实文献，建议通过PubMed或Web of Science使用关键词"MANSC1 recombinant protein"进行检索，并优先选择近5年发表的实验研究论文。
MANSC1 (MANSC domain-containing protein 1) is a relatively understudied protein encoded by the *MANSC1* gene in humans. It belongs to the MANSC family, characterized by a conserved MANSC domain—a structural motif predicted to play roles in metal ion binding or protein-protein interactions, though its precise biochemical function remains unclear. The protein is ubiquitously expressed in various tissues, with higher levels observed in the liver, kidney, and reproductive organs, suggesting potential roles in organ development or homeostasis.
Emerging research links MANSC1 to cellular processes such as adhesion, proliferation, and apoptosis. Studies indicate its involvement in modulating the extracellular matrix (ECM) and influencing signaling pathways like TGF-β, which are critical in tissue repair and fibrosis. Notably, MANSC1 has garnered attention in cancer biology. Dysregulated expression has been reported in malignancies such as hepatocellular carcinoma, breast cancer, and colorectal cancer, where it may act as a tumor suppressor or promoter depending on context. For instance, downregulation of MANSC1 in some cancers correlates with poor prognosis, potentially due to enhanced metastasis or therapy resistance.
Recombinant MANSC1 protein is typically produced using bacterial or mammalian expression systems, often fused with tags (e.g., His-tag) for purification. Its applications span structural studies, interaction assays, and functional investigations in vitro or in animal models. Researchers also utilize it to generate antibodies for diagnostic or therapeutic exploration. Despite progress, key questions persist regarding its molecular mechanisms, interaction partners, and therapeutic potential. Further studies are needed to elucidate its role in both physiological and pathological conditions, particularly in cancer progression and tissue remodeling.
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